Analytical Characterization Services
Comprehensive structural, purity, and DAR analysis using state-of-the-art instrumentation. Know exactly what you made before moving to the next stage.
Full Analytical Menu
Every analysis is performed by experienced analytical scientists and delivered in a clear, report-ready format. We can run characterization on conjugates produced by iQ Biosciences or on material you provide.
| Analysis | Method | What It Tells You |
|---|---|---|
| Drug-Antibody Ratio (DAR) | HIC, RP-HPLC, LC-MS (intact mass) | Average DAR, DAR distribution, conjugation efficiency |
| Purity / Aggregation | SEC-HPLC | Monomer %, aggregate %, fragment content |
| Unconjugated Antibody / Free Drug | RP-HPLC, HIC | Residual unconjugated species, process impurities |
| Intact Mass Analysis | Q-TOF LC-MS | Molecular weight confirmation, payload attachment |
| Peptide Mapping | Q-TOF LC-MS (bottom-up) | Conjugation site identification, sequence coverage |
| Hydrophobicity Profile | HIC (Hydrophobic Interaction Chromatography) | DAR species separation, hydrophobicity impact of payload |
| Plasma Stability | LC-MS bioanalysis (human or mouse plasma) | Linker stability, payload release kinetics in plasma |
| Lysosomal Release | Cell-based lysosomal assay | Payload release in lysosomal compartment |
Instrumentation
Our analytical platform is built around the same instruments used in leading ADC development programs worldwide.
Agilent 1290 Infinity III UHPLC
Ultra-high performance liquid chromatography for RP-HPLC, HIC, and SEC analyses. Delivers high-resolution separation and reproducible results.
Agilent 6530 Q-TOF LC-MS
Quantitative time-of-flight mass spectrometry for intact mass analysis, peptide mapping, and DAR determination by mass. Industry-standard for ADC characterization.
HIC (Hydrophobic Interaction Chromatography)
Separates ADC species by DAR value based on hydrophobicity. Essential for visualizing DAR distribution and assessing conjugation homogeneity.
SEC-HPLC (Size Exclusion)
Quantifies monomer content, aggregates, and fragments. A standard release test for any ADC or biologic product.
Endosafe® Endotoxin Testing
Rapid endotoxin quantification using the Endosafe® system (cartridge-based LAL assay). Ensures ADCs and bioconjugates meet endotoxin thresholds for in vitro assays and in vivo studies.
