In Vitro Bioassay Menu
Functional characterization assays to confirm your ADC’s potency, specificity, mechanism of action, and stability in biologically relevant systems.
Available Assays
All assays are developed and executed by our in-house immunology scientists. We tailor each assay to your specific ADC, target, and cell line — no off-the-shelf kits, no guesswork.
ADC Plasma Stability Assay
Incubate your ADC in human or mouse plasma and monitor payload release over time by LC-MS. Provides critical in vitro PK data before committing to in vivo studies.
Lysosomal Release Assay
Cell-based assay confirming that your cleavable linker releases payload in the lysosomal compartment after internalization. Key mechanistic validation for pH-sensitive or cathepsin-cleavable linkers.
Kupffer Cell Uptake Assay
In vitro assessment of ADC uptake by liver-resident macrophages (Kupffer cells). Provides early insight into clearance risk, Fc-mediated interactions, and potential impact on pharmacokinetics.
Target Binding Confirmation
ELISA and/or flow cytometry to confirm that conjugation has not impaired antigen or target cell binding affinity or specificity. Run pre- and post-conjugation for direct comparison.
ADC Internalization Assays
Quantify receptor-mediated internalization kinetics in antigen-positive and antigen-negative cell lines, via flow cytometry.
Evaluation of Effector Function
Evaluate ADCC, ADCP, and CDC activity of your ADC or unconjugated antibody using primary human immune effector cells or complement, alongside direct Fc receptor binding. Critical for understanding Fc-mediated and non-cytotoxic mechanisms of action.
Direct Cytotoxicity Assays
Dose-response cytotoxicity in antigen-positive and antigen-negative cell lines to determine IC50, maximum killing, and on-target selectivity of your ADC.
Bystander Effect Co-Culture Assays
Evaluate bystander killing of antigen-negative cells in co-culture with antigen-positive target cells. Particularly relevant for membrane-permeable payloads and heterogeneous tumor models.
Custom Assay Development
Don’t see what you need? Our scientists can develop custom bioassays tailored to your specific target biology, cell line, or readout requirement. Contact us to discuss your project.
