C1q Binding Immunoassay
The expert immunologists at iQ Biosciences perform C1q binding immunoassays to assess therapeutic antibody interactions with the complement system, specifically C1q protein. These assays provide early insights into potential complement-mediated immune responses. Our data support drug development by helping clients confirm the presence or absence of complement engagement, ensuring informed decision-making in antibody engineering and therapeutic design.
Our services
- Assess the binding of your therapeutic to human C1q protein, the initiator of the classical complement pathway.
- Utilize commercially available AlphaLISA reagents to measure human C1q binding to the Fc region of IgG antibody therapeutics, with a fluorescence-based plate reader readout.
- Compatible with chimeric, humanized, and fully human antibody therapeutics, including IgG1, IgG2 and IgG4 isotypes.
- Support and enhance data from cell-based functional bioassays, such as Complement-Dependent Cytotoxicity (CDC).
- Generate highly sensitive binding data to complement characterization and safety assessment, supporting IND submission for your therapeutic.
The iQ Experience
Tailored Experiments
Your research is important to us. Our scientists will work through each step of the process with you, including assay design, data analysis, and recommendations for future studies.
Streamlined Process
Simplify your workflow. Bypass the middle-man: at iQ Biosciences, you’ll get immediate access to our biospecimen inventory, saving you both valuable time and money.
Expertise
We’re experts – so you don’t have to be. Augmenting years of experience in immunology and working with immune assays, our scientists stay current with the latest publications and technology.
Exceptional Service
We’re here to help. We know the challenges you’re facing: whether it be through expedited service or our complimentary consulting services, our team is dedicated to helping you reach your goals.
Service overview
How can iQ Biosciences help achieve your goals the smarter way?
C1q and Complement
C1q is the initiating component of the classical pathway of the complement system, a key part of the immune response. C1q recognizes and binds to the Fc region of antibody-antigen complexes, as well as directly to certain pathogens, activating the complement cascade. This activation leads to C3 convertase formation, driving inflammation, opsonization (marking pathogens for destruction), and the assembly of the membrane attack complex (MAC), which can directly lyse target cells. Depending your therapeutic’s mechanism of action and target disease, it may be critical to confirm C1q recognition via binding or demonstrate C1q evasion for safety assessment.
Mechanism of Assay
The AlphaLISA method for detecting human C1q binding to the antibody Fc region utilizes a biotinylated anti-human IgG Fab antibody, which binds to Streptavidin Donor beads. These beads capture and aggregate the IgG antibody present in the sample. Simultaneously, AlphaLISA anti-C1q Acceptor beads bind C1q protein via its collagen-like domain. When the antibody binds to C1q, the Donor and Acceptor beads are brought into proximity. Upon excitation of the Donor beads, they generate singlet oxygen, initiating an energy transfer cascade within the Acceptor beads. This results in a distinct light emission peak at 615 nm.
A
In this assay, positive signal correlates with C1q binding. We utilize Rituxan and IgG4 isotype as positive and negative (reduced binding) controls in this assay, respectively. (A) Example data of Test Article A showing minimal C1q binding in reference to assay positive and negative controls.
Check out our Fcγ receptor binding assays, commonly conducted alongside our C1q binding immunoassay for a comprehensive assessment of antibody function.
Order Information
Pricing
At iQ Biosciences, we understand that each client is unique. Upon quote request, we will provide complimentary consulting services to determine how to tailor our services to best meet your goals. Based on this preliminary assessment, we will be able to provide a price quote.
Expected Timetable
Fit a timetable appropriate to your research goals. We work with you to determine your expected turn-around on a case-by-case basis and ensure that our services fit your needs. Some experiments may require an expedited service – please inquire for more information.
