- Evaluate induction of target cell death by a therapeutic of interest with an in vitro apoptosis assay.
- Apoptosis by flow cytometric analysis is determined against your target cell type of interest with dye conjugates that specifically target apoptotic cells (e.g., Annexin V).
- Direct or cross-linking induced apoptosis assays offered.
- Assay controls to account for background cell death are included. A positive control and various concentrations of your therapeutic of interest are included to elucidate any dose-responsive effects.
- Data are provided in appropriate formats, including PowerPoint slides, Excel files, and/or direct data files (e.g., GraphPad Prism).
Service Overview
Service overview
One hallmark of cancer cells is their ability to evade cell death. Therefore, targeting apoptotic cell death pathways to make cancer cells more susceptible to cell death is an attractive option for immunotherapy. At iQ Biosciences, we can screen therapeutics for their potential to cause apoptosis of target cells in vitro, helping you to determine which candidates are most effective. We use a flow cytometry-based approach to measure the degree of apoptosis in response to a therapeutic of interest alongside positive (e.g., staurosporine) and negative (e.g., isotype control antibody) controls.
Direct apoptosis of the target cell type of interest is measured via flow cytometry analysis of fluorescently-labeled Annexin V binding. Annexin V binds to phosphatidylserine, a phospholipid that is trafficked to the outer membrane of cells during early apoptosis. Propidium iodide is also used to identify late apoptotic or necrotic cells.
Cross-linking-induced apoptosis can also be assessed with the addition of an appropriate secondary antibody during incubation. The percentage of Annexin V+ apoptotic cells are reported and plotted with regression curves to elucidate the maximum % apoptosis and EC50 values of each therapeutic candidate. Pilot studies to identify optimal apoptosis conditions and controls for the target cells of interest can be developed and performed upon request.
The iQ Experience
Tailored Experiments
Your research is important to us. Our scientists will work through each step of the process with you, including assay design, data analysis, and recommendations for future studies.
Streamlined Process
Simplify your workflow. Bypass the middle-man: at iQ Biosciences, you'll get immediate access to our biospecimen inventory, saving you both valuable time and money.
Expertise
We're experts- so you don't have to be. Augmenting years of experience in immunology and working with immune assays, our scientists stay current with the latest publications and technology.
Exceptional Service
We're here to help. We know the challenges you're facing: whether it be through expedited service or our complimentary consulting services, our team is dedicated to helping you reach your goals.
