- Determine the cytotoxicity of your engineered CAR T-cells against target cells of interest, or against off-target tissue representative cell lines, with an in vitro CAR T cytotoxicity assay.
- A range of CAR T: target cell ratios can be assessed in co-culture assays to elucidate CAR T cytotoxic potency and to determine which conditions are ideal for maximal CAR T activity.
- CAR T-dependent cytotoxicity of target cells is determined via flow cytometric analysis.
- Co-culture supernatants can be harvested and stored for optional downstream multiplexed cytokine quantitation.
Service Overview
About the CAR T-Cell Cytotoxicity Assay
The first chimeric antigen receptor (CAR) T-cell therapy was approved by the FDA in 2017 and CAR T therapies have since shown significant promise for long-term remission in several types of cancers. The FDA released draft guidance documents encouraging researchers to take initiative to evaluate CAR T-cell biological activity in the early stages of CAR T-cell development. CAR T-cell cytotoxicity assays performed at iQ Biosciences can help researchers evaluate their CAR T cellular therapy from both a safety and efficacy perspective. The resulting data can inform starting doses and help researchers screen CAR T candidates to assess.
To assess CAR T cytotoxicity, target cells of interest are labeled with a fluorescent cell trace dye and co-cultured with CAR T cells or control effector cells at several ratios to determine any cell ratio-dependent effects. After incubation, the cells are harvested, stained with a fluorescent viability dye, and acquired via high-throughput flow cytometry, to determine the CAR T-cell-dependent specific lysis of target cells. Co-culture supernatants can be harvested and utilized for cytokine measurement, also performed at iQ Biosciences.
The iQ Experience
Tailored Experiments
Your research is important to us. Our scientists will work through each step of the process with you, including assay design, data analysis, and recommendations for future studies.
Streamlined Process
Simplify your workflow. Bypass the middle-man: at iQ Biosciences, you'll get immediate access to our biospecimen inventory, saving you both valuable time and money.
Expertise
We're experts- so you don't have to be. Augmenting years of experience in immunology and working with immune assays, our scientists stay current with the latest publications and technology.
Exceptional Service
We're here to help. We know the challenges you're facing: whether it be through expedited service or our complimentary consulting services, our team is dedicated to helping you reach your goals.
