Mixed Lymphocyte Reaction (MLR) Assay

Mixed Lymphocyte Reaction (MLR) Assay2020-11-15T01:20:10-08:00
  • Perform one- and two-way MLR assays with human and mouse cells
  • Variety of functional readouts available, including proliferation, cytokine production, and cytotoxicity
  • Personalized experimental design to ensure the most biologically relevant data is obtained
  • All assays are performed with high quality reagents and genotyped samples
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Service Overview

About iQ Biosciences’ Mixed Lymphocyte Reaction (MLR) Assay Service

Major histocompatibility complexes are some of the most diverse haplotypes in the human and mouse genome. The function of these molecules is to present peptides processed from ingested full length proteins or internal proteins on antigen presenting cells (APCs). The ligand for the peptide/MHC complex is the T cell receptor (TCR) on T cells, which becomes activated if the TCR binds the peptide/MHC complex.

Besides the ability to present peptides, MHC molecules are extremely important for T cell development, which occurs in the thymus. There, self peptides, which are generated from innate proteins, are presented on MHC molecules to developing T cells. T cells whose TCRs bind the self-peptide/MHC complexes tightly are deleted (negative selection), while those that have no affinity die from the lack of signals. In contrast, TCRs that bind in moderate strength are selected (positive) to exit into the periphery to combat foreign pathogens. This selection process is highly dependent on the individual’s MHC molecule in which the TCR and its T cell will not recognize the peptide presented on another MHC haplotype.

In early studies of T cell development and function, scientists noticed that the splenocytes from the F1 progeny of parents with different haplotypes did not react to irradiated (non-functional, but still presented peptide/MHC) splenocytes from either parental background. However, if splenocytes from either parent were mixed with irradiated F1 splenocytes, the parental T cells responded as they proliferated, produced cytokine, and promoted cytotoxicity.

Further studies dissected the basis for these reactions, and it was discovered to be based on the difference in the MHC haplotype. Because the F1 APCs expressed MHC molecules from both parental strains, the F1 T cells were selected on both of them so the T cells would recognize those MHC molecules as self and not react. In contrast, T cells from the parental strain would respond to F1 splenocytes because the APCs express both MHC haplotypes and the parental T cells would recognize the other parent’s haplotype as foreign and react to it. These types of experiments are now commonly known as mixed lymphocyte reaction (MLR) assays and used to interrogate T cell function.

iQ’s MLR Assays: Designed with Your Goals in Mind

At iQ Biosciences, we have experience performing MLR assays and will work with you to design experiments relevant to your studies. Our MLR cultures consist of co-culturing isolated CD4 cells with in vitro differentiated DCs of a different background. After the culture period, proliferation and cytokine production are often assessed. For proliferation, iQ offers a variety of methods including CellTiter-Glo and CFSE dilution while cytokine production is measured with Cytokine Bead Arrays or AlphaELISA for supernatant-based readouts.

Therapeutic molecules, such as those that target immune checkpoint proteins, can also be added to the MLR assay to determine their effect on T cell function. For example, PD-1 checkpoint inhibitors can be included in cultures followed by assessment of IFN-g production. Other functional readouts can also be performed to corroborate the observations.

With our extensive knowledge of factors that influence MLRs and our flexible readouts, iQ can help you design the right experiments for your studies. Please contact us for more information or request a free quote.

The iQ Experience

Tailored Experiments

Your research is important to us. Our scientists will work through each step of the process with you, including assay design, data analysis, and recommendations for future studies.

Streamlined Process

Simplify your workflow. Bypass the middle-man: at iQ Biosciences, you'll get immediate access to our biospecimen inventory, saving you both valuable time and money.

Expertise

We're experts- so you don't have to be. Augmenting years of experience in immunology and working with immune assays, our scientists stay current with the latest publications and technology.

Exceptional Service

We're here to help. We know the challenges you're facing: whether it be through expedited service or our complimentary consulting services, our team is dedicated to helping you reach your goals.

Order Information

Pricing

At iQ Biosciences, we understand that each client is unique. Upon quote request, we will provide complimentary consulting services to determine how to tailor our services to best meet your goals. Based on this preliminary assessment, we will be able to provide a price quote.

Expected Timetable

Fit a timetable appropriate to your research goals. We work with you to determine your expected turn-around on a case-by-case basis and ensure that our services fit your needs. Some experiments may require an expedited service – please inquire for more information.