T cell-Dependent Cellular Cytotoxicity (TDCC) Assay
The immunology experts at iQ Biosciences perform T cell-dependent cellular cytotoxicity (TDCC) assays to evaluate the potency of T-cell engagers (TCEs) and other T cell-redirecting therapeutics. Using flow cytometry- and plate reader-based approaches, we assess target cell viability and depletion at defined effector-to-target (E:T) ratios. With appropriate controls and dose-dependent analysis, our TDCC assay provides high-quality data to support TCE screening, lead optimization, and preclinical development.
Our services
- Determine the efficacy of your T-cell engager (TCE), or other, therapeutic with an in vitro T cell-dependent cellular cytotoxicity assay
- High quality cryopreserved Human and Cynomolgus Monkey T cells used as effector cells, isolated and validated at iQ Biosciences
- T-cell dependent target cell cytotoxicity is assessed via flow cytometric or plate-reader based measurements
- All assays are performed with high-quality reagents and strict controls by experienced immunologists.
- Data are provided in appropriate formats, including PowerPoint slides, Excel files, and/or direct data files (e.g., GraphPad Prism).
The iQ Experience
Tailored Experiments
Your research is important to us. Our scientists will work through each step of the process with you, including assay design, data analysis, and recommendations for future studies.
Streamlined Process
Simplify your workflow. Bypass the middle-man: at iQ Biosciences, you’ll get immediate access to our biospecimen inventory, saving you both valuable time and money.
Expertise
We’re experts – so you don’t have to be. Augmenting years of experience in immunology and working with immune assays, our scientists stay current with the latest publications and technology.
Exceptional Service
We’re here to help. We know the challenges you’re facing: whether it be through expedited service or our complimentary consulting services, our team is dedicated to helping you reach your goals.
Service Overview
How can iQ Biosciences help achieve your goals the smarter way?
T-cell Engagers (TCEs) have gained popularity in the field of immunotherapy due to their ability to dually interact with two antigens of interest, often redirecting T cell effector function via CD3 toward cancer cells expressing a tumor antigen.
At iQ Biosciences, our T cell-dependent cellular cytotoxicity (TDCC) assay can help you screen candidate TCEs, including bispecific antibodies (e.g., BiTEs, DARTs, and TandAbs), TCR-based therapeutics, and other T-cell-redirecting biologics,, for effective cytotoxicity against target cell(s) of interest. Target cells, typically cancer cell lines, are labeled with a cell trace dye and then co-cultured Pan T (CD3+) cells previously isolated from whole blood obtained from healthy donors. Assays are performed at defined effector:target (E:T) ratios and timepoints, depending on relevant factors. A range of therapeutic concentrations are tested to elucidate any dose-dependent cytotoxicity. We also provide or recommend appropriate positive controls (recombinant versions of FDA-approved BiTE antibodies, for example, as available) and negative controls to ensure assay validity and accuracy. After incubating the co-culture with therapeutic molecules or controls, viability and/or depletion of target cells is determined by flow cytometric or plate-reader based analysis.
This assay can be combined with cytokine quantitation or T cell activation marker analysis, to provide additional functional insights. In addition to Human T cell TDCC assays, we offer Cynomolgus Monkey (cyno) TDCC assays.
Order Information
Pricing
At iQ Biosciences, we understand that each client is unique. Upon quote request, we will provide complimentary consulting services to determine how to tailor our services to best meet your goals. Based on this preliminary assessment, we will be able to provide a price quote.
Expected Timetable
Fit a timetable appropriate to your research goals. We work with you to determine your expected turn-around on a case-by-case basis and ensure that our services fit your needs. Some experiments may require an expedited service – please inquire for more information.
