Immune Monitoring

Immune Monitoring 2018-05-30T01:25:47+00:00
  • Evaluate your small molecule, biologic, or formulation excipients for its potential to bind or activate primary cells from multiple species (human, non-human primate, rodent)
  • We routinely perform immunophenotyping of in vitro or in vivo (safety, PD, xenograft, or clinical studies) treated samples (cell lines or primary cells) for specific protein target expression on cell subsets or biomarker detection indicative of bioactivity (cell surface activation markers, cytokine detection)
  • Personalized experimental design to ensure the most biologically relevant data is obtained
  • These assays are applicable to multiple species (human, non-human primates, & rodents) for cross-reactivity assessment of your molecules
  • All assays are performed with high-quality reagents and strict controls by experienced immunologists
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Service Overview

About Immune Monitoring

There has been a recent emphasis on the development of therapeutics that modulate the immune system to treat oncology indications. Unfortunately, one of the major obstacles to effective cancer immune therapy is the lack of available immune monitoring (World Immunotherapy Council Recommendations, Journal of Translational Medicine 2011). There is currently no consensus on what immune tests should be ordered to monitor treatment and response (ISBTc-SITC/FDA/NCI Workshop Clinical Cancer Research 2011).

At iQ Biosciences, we believe that immune monitoring must be personalized to the type of therapy given.  Therefore, we can provide these services by offering a variety of assays to characterize the immune status and makeup of patients receiving immune modulatory therapeutics.  With our experienced immunologists, we aim to provide tailored consultation to help identify the best immunologic assays for your clinical trial based on a detailed consultation.

Immune Monitoring with iQ Biosciences

The assays we offer include:

  • Characterization of normal cell populations (B/T cells, NK cells, macrophages, etc.) and rare populations (Foxp3 Tregs, iNKT, DCs, etc.) by flow cytometry
  • Characterization of the activation status of immune populations based on surface markers or intracellular cytokine staining by flow cytometry
  • Characterization of soluble molecules, such as cytokines and antibodies, by cytometric bead assays or ELISA
  • Characterization of antigen specific cells
  • Cytotoxic capabilities of immune cells (please also see our pages on ADCC and CDC assays)

As an example of our services, we can provide flow cytometric analysis of immune cell types from peripheral blood cells, as well as measure cytokines in peripheral blood plasma from a single 10-milliliter sample. In addition, we have standard panels of markers dedicated to elucidating activation status of various immune cells (see table below). However, we can design custom panels of markers specific for your studies.

 Cell Type
T cellTreg cellB cellDC/mono/NK cellsTh1/2/17 cells

Markers
CD3
CD4
CD8
CD38
CD45RA
CCR7
HLA-DR
CD3
CD4
CD25
CD45RO
CD127
CCR4
HLA-DR
CD3
CD19
CD20
CD24
CD27
CD38
IgD
CD3
CD11c
CD14
CD16
CD19
CD20
CD56
CD123
HLA-DR
CD3
CD4
CD8
CD38
CCR6
CXCR3
HLA-DR

Table 1: iQ Bioscience’s standard panel of markers for phenotypical analysis of immune cells.

After data collection for any of the assays, our experts will analyze the data and provide a summary of the findings. If needed, we can provide the primary LIS mode files for you to assemble dot plots for publications / presentations. We can also make recommendations for future studies.

Our team at iQ Biosciences has numerous years of experience with immunology and we believe that working with you every step of the way ensures an optimal experimental design to answer your scientific questions. Given the current trend towards immune-modulating molecules, we will provide personalized service and expertise to help you accomplish your goals.

The iQ Experience

Tailored Experiments

Your research is important to us. Our scientists will work through each step of the process with you, including assay design, data analysis, and recommendations for future studies.

Streamlined Process

Simplify your workflow. Bypass the middle-man: at iQ Biosciences, you'll get immediate access to our biospecimen inventory, saving you both valuable time and money.

Expertise

We're experts- so you don't have to be. Augmenting years of experience in immunology and working with immune assays, our scientists stay current with the latest publications and technology.

Exceptional Service

We're here to help. We know the challenges you're facing: whether it be through expedited service or our complimentary consulting services, our team is dedicated to helping you reach your goals.

Order Information

Pricing

At iQ Biosciences, we understand that each client is unique. Upon quote request, we will provide complimentary consulting services to determine how to tailor our services to best meet your goals. Based on this preliminary assessment, we will be able to provide a price quote.

Expected Timetable

Fit a timetable appropriate to your research goals. We work with you to determine your expected turn-around on a case-by-case basis and ensure that our services fit your needs. Some experiments may require an expedited service – please inquire for more information.