Simple Western
Simple Western is an automated, capillary-based protein analysis service that brings your laboratory fast, quantitative, and highly reproducible Western blot results. Unlike traditional Western blots, Simple Western streamlines the entire process—automating protein separation, immunodetection, washing, and data analysis within a single instrument. With this technology, precise protein quantification, characterization, and post-translational modification detection with minimal hands-on time and ultra-low sample requirements.
Our services
- Automated, capillary-based protein analysis for fast and reproducible Western blot results.
- Requires minimal sample input and offers high sensitivity—ideal for preclinical studies with limited material.
- Supports multiplex protein detection, post-translational modification analysis, and total protein normalization.
- Provides quantitative and highly consistent data for protein expression, isoform differentiation, and signaling pathway studies.
- Customized assay design and rapid turnaround times to advance your preclinical research with confidence.
The iQ Experience
Tailored Experiments
Your research is important to us. Our scientists will work through each step of the process with you, including assay design, data analysis, and recommendations for future studies.
Streamlined Process
Simplify your workflow. Bypass the middle-man: at iQ Biosciences, you’ll get immediate access to our biospecimen inventory, saving you both valuable time and money.
Expertise
We’re experts – so you don’t have to be. Augmenting years of experience in immunology and working with immune assays, our scientists stay current with the latest publications and technology.
Exceptional Service
We’re here to help. We know the challenges you’re facing: whether it be through expedited service or our complimentary consulting services, our team is dedicated to helping you reach your goals.
Service overview
How can iQ Biosciences help achieve your goals the smarter way?
Our Simple Western service supports small sample volumes and is ideal for preclinical projects where sensitivity, throughput, and consistency matter most. We offer multiplex protein detection, total protein normalization, and can resolve size-based assays, providing detailed insights into protein expression, isoforms, and signaling pathways—even in complex biological samples such as whole-cell lysates or tissue homogenates.
By entrusting us with your Simple Western needs, you benefit from rapid turnaround, transparent analysis, and customizable assay design tailored to your research questions. Our experienced team ensures your protein science projects move forward efficiently, with the data you need to make confident, data-driven decisions in preclinical research and drug development.
Simple Western generates data in two primary formats: the electropherogram (peak view) and the (lane view). The peak view presents protein detection as quantitative peaks on an electropherogram, where the area under each peak corresponds directly to protein abundance—enabling precise, automated quantification free from subjective interpretation. The digital band view mimics traditional Western blot bands but in an electronic, highly reproducible format, offering a familiar visualization for quick assessment of protein size and presence across samples. Both views are automatically produced, ensuring clear, publication-ready results that facilitate easy data interpretation and robust comparison between experiments.
We have sterile tissue culture facilities if cell culture, treatments, and sample collection are needed. Please specify your needs when requesting a quote!
Expression Assessment
Simple Western is an invaluable platform for assessing protein expression following genetic modifications such as gene knock-in, knock-out, or overexpression studies. Its automated and quantitative capabilities enable precise detection of target proteins at low abundance, even from minimal sample material. This allows for efficient verification of genetic edits, confirmation of on- or off-target effects, and side-by-side comparison of parental and modified cell lines. The digital readout provides clear, publication-quality data, supporting reliable evaluation of genetic engineering outcomes in immunology research.
Total Protein Degradation Assay
Small molecule degraders, such as PROTACs (Proteolysis Targeting Chimera), molecular glues, and monovalent degraders, often require screening and confirmation of target protein degradation. Simple Western provides a streamlined and sensitive solution to quantify target protein degradation from in vitro cell lysates or in vivo tissue samples. By enabling high-throughput analysis with minimal protein input, it allows researchers to quickly assess PROTAC potency across multiple compounds or concentrations. The system’s quantitative output ensures accurate measurement of residual target protein, making it ideal for establishing dose-response curves and comparing degradation efficiency in various cell types or experimental settings.
Cell Signaling Activity
Simple Western excels at monitoring cell signaling dynamics, including phosphorylation events critical to immune cell function. Its multiplexing ability enables simultaneous analysis of both total and phosphorylated forms of proteins in time course experiments. This allows for detailed assessment of pathway activation and signal transduction kinetics following stimulation or drug treatment. Researchers gain robust, reproducible insights into signaling cascades, facilitating a deeper understanding of immune regulatory mechanisms and drug action.
Order Information
Pricing
At iQ Biosciences, we understand that each client is unique. Upon quote request, we will provide complimentary consulting services to determine how to tailor our services to best meet your goals. Based on this preliminary assessment, we will be able to provide a price quote.
Expected Timetable
Fit a timetable appropriate to your research goals. We work with you to determine your expected turn-around on a case-by-case basis and ensure that our services fit your needs. Some experiments may require an expedited service – please inquire for more information.
