Antibody-Dependent Cell-Mediated Cytotoxicity

What is ADCC and why would you need it? This is Part 2 of our series, “ADCC: The Antibody Awakens…the Immune System”. Miss Part 1? Catch up here. Potential Applications & Implications for ADCC assays As alluded to in Part 1, one of the most important applications for ADCC assays is to identify candidate therapeutic antibodies that can mediate ADCC activity. Originally thought to prevent downstream signaling of the target receptor by blocking its oligomerization or engagement to ligands, most therapeutic antibodies are now suggested to work through multiple mechanisms, including ADCC. Thus, many companies screen their therapeutic antibody candidates [...]

What is ADCC and why would you need it? What is Antibody-Dependent Cell-Mediated Cytotoxicity? Antibody-dependent cell-mediated cytotoxicity (ADCC) is the lysis of target cells mediated by antibodies that recruit effector cells to the target. This is achieved when the antigen recognition domain of the antibody binds antigen on the target, and the Fc domain of the antibody binds Fc receptors on effector cells. This engagement of Fc receptors results in activation of the effector cells and the subsequent release of cytolytic molecules towards the target to promote lysis. What are the molecules and effector cell types involved in ADCC? Several [...]