Antibody-Dependent Cell-Mediated Cytotoxicity

Serious Monkey Business: A Short Take on Cynomolgus Monkeys in Research

What are cynomolgus monkeys? Cynomolgus monkeys (Macaca fascicularis), also known as the long-tailed or crab-eating macaque, are non-human primates (NHP) commonly used in biomedical research.  There are 10 subspecies of these macaques and they are found predominantly in southeast Asia.  The cynomolgus monkeys are typically 15-22 inches long, and the females weigh between 7-13 pounds, while males can weigh between 11-20 pounds. Why use cynomolgus monkeys and how are they used in biomedical research? Cynomolgus monkeys are frequently used in biomedical research because researchers believe these monkeys are the ideal models due to the 90-93% genetic similarity to and recent [...]

ADCC: The Antibody Awakens…the Immune System (Part 2)

What is ADCC and why would you need it? This is Part 2 of our series, “ADCC: The Antibody Awakens…the Immune System”. Miss Part 1? Catch up here. Potential Applications & Implications for ADCC assays As alluded to in Part 1, one of the most important applications for ADCC assays is to identify candidate therapeutic antibodies that can mediate ADCC activity. Originally thought to prevent downstream signaling of the target receptor by blocking its oligomerization or engagement to ligands, most therapeutic antibodies are now suggested to work through multiple mechanisms, including ADCC. Thus, many companies screen their therapeutic antibody candidates [...]

ADCC: The Antibody Awakens…the Immune System (Part 1)

What is ADCC and why would you need it? What is Antibody-Dependent Cell-Mediated Cytotoxicity? Antibody-dependent cell-mediated cytotoxicity (ADCC) is the lysis of target cells mediated by antibodies that recruit effector cells to the target. This is achieved when the antigen recognition domain of the antibody binds antigen on the target, and the Fc domain of the antibody binds Fc receptors on effector cells. This engagement of Fc receptors results in activation of the effector cells and the subsequent release of cytolytic molecules towards the target to promote lysis. What are the molecules and effector cell types involved in ADCC? Several [...]

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