Name: Cynomolgus Monkey Peripheral Blood Mononuclear Cells (PBMCs)
SKU: IQB-MnPB1xx
Availability: InStock

Cynomolgus Monkey Peripheral Blood Mononuclear Cells (PBMCs)

$620.00 – $845.00

High-quality cynomolgus macaque PBMCs sourced responsibly from a vetted third party facility
High viability (> 90%) after thawing
Used for a wide variety of safety assessment and functional assays
All orders come with an iQ Certificate of Analysis
Normally ships out same business day

SKU: IQB-MnPB1xx Categories: Cynomolgus, Monkey, PBMC Tags: cyno, cynomolgus, monkey, pbmc

Description

About the Cynomolgous Monkey (or Cynomolgous Macaque)

The cynomolgous macaque is the most commonly utilized non-human primate in biomedical research. According to some researchers, these monkeys are ideal models due to the 90-93% genetic similarity to and recent evolutionary divergence from humans. They are employed in numerous research areas, such as immunology, neuroscience, oncology, diabetes, and pharmacology due to their genetics and physiology.

Application Summary for Cynomolgus Monkey PBMCs

Cynomolgus monkey PBMCs (or Cyno PBMCs) can be used for a wide variety of safety assessment and functional studies. They are commonly employed in pre-clinical settings to ensure biologics are not eliciting unwanted functions, such as cytokine release and toxicity. They are also used in many immunology-based applications, such as ELISPOT, cytokine production assays, and cytotoxicity assays to measure ADCC or CDC in response to biologics. In addition, cynomolgus monkey PBMCs can also be used in ex-vivo applications for cell population characterization.

Isolation of PBMCs

Peripheral blood is obtained from responsible facilities that have many years of experience collecting samples. Since the facilities are local, the blood is quickly transported to iQ Bioscience’s laboratory for processing.

Once at our lab, PBMCs are isolated by performing density centrifugation with Ficoll, a high molecular weight sucrose solution, to remove red blood cells under sterile conditions . Depending on the species (human or non-human) of blood, either 1.077 or 1.084 g/ml Ficoll is used, resulting in a perfect layer of mononuclear cells that are separated from plasma, platelets, granulocytes, and erythrocytes. Additional quality control steps are taken to isolate and prepare PBMCs that ensure the highest viability for cryopreservation and downstream experimental applications.

Cryopreservation and Storage

Our cyno PBMCs are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 90%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).

Quality Control Process

All of our non-human primate donors have been tested for Herpes B virus, Simian Retrovirus (SRV), and tuberculosis. Additionally, we implement random sampling per lot to test for viability and cell counts to ensure they meet specifications, which are recorded on the Certificate of Analysis that is included with each shipment. Cell counts are obtained using a manual hemocytometer and then cross-referenced with an automated cell counter. Each lot is also characterized for unique cell populations by immunophenotyping (Figure 1), in which the results are also recorded on the Certificate of Analysis.

Figure 1. Flow profiles of T cells (CD3, CD4 gated on CD3+ population, and CD8 gated on CD3+ population), monocyte (CD14), B cell (CD20), and NK cell (CD56) populations in cyno PBMCs. The specific immune cell subset and its percentage is listed on the top right of the flow profile. Briefly, cyno PBMCs were stained with antibodies for CD3, CD4, CD8, CD14, CD20, and CD56, followed by flow cytometric analysis.

Additional information

Available Size(s)	10 million cells / vial, 10 million cells / vial, monocytes depleted, 5 million cells / vial
Format	Frozen
Cell Type	Mononuclear
Tissue Type	Normal Peripheral Blood
Species	Monkey
Viability	> 90%

We are happy to accommodate the following donor requests if inventory is available. Feel free to inquire prior to ordering, or specify your interest in the "Order notes" section during checkout, and we will follow-up with you shortly after your order is processed, if necessary:

Single or multiple donors for a particular product (SKU). Indicate the number of vials from each individual donor
Donor(s) with a higher percentage of the cells of interest (e.g., B cells, CD4+ cells, CD8+ cells, NK cells, etc.)

We are Committed to Ethical Practices

iQ Biosciences’ primary cell products from non-human primates are collected under approved IACUC protocol, which was developed in consultation with the Attending Veterinarian and is consistent with current veterinary standards. The collection facility is AAALACi and PHS accredited, and all animal housing, handling, and research protocols are consistent with standards set forth by the Animal Welfare Act, NIH's Policy on Humane Care and Use of Animals and the Guide for the Care and Use of Animals. All animal housing and handling is done in such a way to minimize stress and risk of injury to the animal.

For US customers, we ship via FedEx Overnight Shipping. Shipping charges will vary per shipping address (based on ZIP code) and are estimated to be $140.

For international (non-US) customers, we work closely with you and our couriers to ensure all necessary documentation is in place for international shipments to significantly reduce the chance of delays at Customs. For the export of non-human primate samples, this includes preparing CITES permits, as well as any other documentation as required by country. Please submit an inquiry to orders@iqbiosciences.com for your estimated time of delivery and shipping charges.

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