Cynomolgus Monkey Peripheral Blood Mononuclear Cells (PBMCs)

Cynomolgus Monkey Peripheral Blood Mononuclear Cells (PBMCs)

$425.00

  • High-quality cynomolgus macaque PBMCs sourced responsibly from a vetted third party facility
  • High viability (> 90%) after thawing
  • Used for a wide variety of safety assessment and functional assays
  • All orders come with an iQ Certificate of Analysis
  • Normally ships out same business day
SKU: IQB-MnPB1xx Categories: , , Tags: , , ,

Description

About the Cynomolgous Monkey (or Cynomolgous Macaque)

The cynomolgous macaque is the most commonly utilized non-human primate in biomedical research. According to some researchers, these monkeys are ideal models due to the 90-93% genetic similarity to and recent evolutionary divergence from humans. They are employed in numerous research areas, such as immunology, neuroscience, oncology, diabetes, and pharmacology due to their genetics and physiology.

Application Summary for Cynomolgus Monkey PBMCs

Cynomolgus monkey PBMCs (or Cyno PBMCs) can be used for a wide variety of safety assessment and functional studies. They are commonly employed in pre-clinical settings to ensure biologics are not eliciting unwanted functions, such as cytokine release and toxicity. They are also used in many immunology-based applications, such as ELISPOT, cytokine production assays, and cytotoxicity assays to measure ADCC or CDC in response to biologics. In addition, cynomolgus monkey PBMCs can also be used in ex-vivo applications for cell population characterization.

Isolation of PBMCs

Peripheral blood is obtained from responsible facilities that have many years of experience collecting samples. Since the facilities are local, the blood is quickly transported to iQ Bioscience’s laboratory for processing.

Once at our lab, PBMCs are isolated by performing density centrifugation with Ficoll, a high molecular weight sucrose solution, to remove red blood cells under sterile conditions . Depending on the species (human or non-human) of blood, either 1.077 or 1.084 g/ml Ficoll is used, resulting in a perfect layer of mononuclear cells that are separated from plasma, platelets, granulocytes, and erythrocytes. Additional quality control steps are taken to isolate and prepare PBMCs that ensure the highest viability for cryopreservation and downstream experimental applications.

Cryopreservation and Storage

Our cyno PBMCs are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 90%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).

Quality Control Process

All of our non-human primate donors have been tested negative for Herpes B virus and Simian Retrovirus (SRV). Additionally, we implement random sampling per lot to test for viability and cell counts to ensure they meet specifications, which are recorded on the Certificate of Analysis that is included with each shipment. Cell counts are obtained using a manual hemocytometer and then cross-referenced with an automated cell counter. Each lot is also characterized for unique cell populations by immunophenotyping (Figure 1), in which the results are also recorded on the Certificate of Analysis.

Immunophenotyping of cyno PBMCs

Figure 1. Flow profiles of T cells (CD3, CD4 gated on CD3+ population, and CD8 gated on CD3+ population), monocyte (CD14), B cell (CD20), and NK cell (CD56) populations in cyno PBMCs. The specific immune cell subset and its percentage is listed on the top right of the flow profile. Briefly, cyno PBMCs were stained with antibodies for CD3, CD4, CD8, CD14, CD20, and CD56, followed by flow cytometric analysis.

Additional information

Available Size(s)

,

Format

Cell Type

Tissue Type

Species

Viability

> 90%

Please let us know in the notes section during checkout if you are interested in animal donor-matched PBMCs, as we carry these in stock.

If you would like to request multiple animal donors or the same animal donor, please specify this in the notes section as well.

Request a Cellular Products Fact Sheet for Non-Human PBMCs

Interested in learning more about this Cellular Product? Complete the form below to request a Fact Sheet.





Tailored Experiments

Your research is important to us. Our scientists will work through each step of the process with you, including assay design, data analysis, and recommendations for future studies.

Streamlined Process

Simplify your workflow. Bypass the middle-man: at iQ Biosciences, you'll get immediate access to our biospecimen inventory, saving you both valuable time and money.

Expertise

We're experts- so you don't have to be. Augmenting years of experience in immunology and working with immune assays, our scientists stay current with the latest publications and technology.

Exceptional Service

We're here to help. We know the challenges you're facing: whether it be through expedited service or our complimentary consulting services, our team is dedicated to helping you reach your goals.

 

For US customers, we ship via FedEx Overnight Shipping. Shipping charges will vary per shipping address (based on ZIP code) and are estimated to be $110.

For international (non-US) customers, we work closely with you and our couriers to ensure all necessary documentation is in place for international shipments to significantly reduce the chance of delays at Customs. For the export of non-human primate samples, this includes preparing CITES permits, as well as any other documentation as required by country. Please submit an inquiry to orders@iqbiosciences.com for your estimated time of delivery and shipping charges.

Fisher Scientific (United States)
Tel: 1-800-766-7000
Web: https://www.fishersci.com

VWR International (United States)
Tel: 1-800-932-5000
Web: https://www.vwr.com

Cosmo Bio Co., Ltd (Japan)
Tel: 03-5632-9610
Fax: 03-5632-9619
Email: nsmail@cosmobio.co.jp
Web: https://www.cosmobio.co.jp

BioClone (South Korea)
Tel: +82-2-2690-0058
Email: bioclone@bioclone.co.kr
Web: http://www.bioclone.co.kr