Cynomolgus Monkey Peripheral Blood Mononuclear Cells (PBMCs)

Description

About the Cynomolgus Monkey (or Cynomolgus Macaque)

The cynomolgus macaque is the most utilized non-human primate in biomedical research. According to researchers, this species of monkey represents an ideal model due to their 90-93% genetic similarity to and recent evolutionary divergence from humans. They are employed in numerous research areas, such as immunology, neuroscience, oncology, diabetes, and pharmacology because of their genetics and physiology.

Application Summary for Cynomolgus Monkey PBMCs

Cynomolgus monkey PBMCs (or Cyno PBMCs) can be used for a wide variety of safety assessments and functional studies. They are commonly employed in preclinical settings to ensure therapeutics are not eliciting unwanted effects, such as cytokine release and toxicity. They are also used in several other immunoassays that we offer as services, such as cytokine release assays, antibody-dependent cellular cytotoxicity (ADCC) assays, immunophenotyping, and immune cell depletion. In addition, Cyno PBMCs can also be used in ex-vivo applications for cell population characterization.

Isolation of PBMCs

Peripheral blood is obtained from responsible facilities that have many years of experience with sample collection. Since the facilities are local, the blood is quickly transported to iQ Biosciences’ laboratory for processing.

PBMCs are isolated using density gradient centrifugation with Ficoll, a high molecular weight sucrose solution. During this process, the enriched layer of mononuclear cells that are separated from plasma, platelets, granulocytes, and erythrocytes, and any resulting erythrocytes are gently lysed to ensure high sample purity. PBMCs are assessed for counts and viability, then carefully cryopreserved to ensure the highest viability upon thaw for downstream experimental applications. See “Cryopreservation and Storage” for more details.

Cryopreservation and Storage

Our Cyno PBMCs are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 90%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).

Quality Control Process

All of our non-human primate donors have been tested for Herpes B virus, Simian Retrovirus (SRV), and tuberculosis. Additionally, we implement random sampling per lot to test for viability and cell counts to ensure they meet specifications, which are recorded on the Certificate of Analysis that is included with each shipment. Cell counts are obtained using a manual hemocytometer and then cross-referenced with an automated cell counter. Each lot is also characterized for unique cell populations by immunophenotyping (Figure 1), in which the results are also recorded on the Certificate of Analysis.

We are happy to accommodate the following donor requests if inventory is available:
Single or multiple donors for a particular product (SKU). Indicate the number of vials from each individual donor
Donor(s) with a higher percentage of the cells of interest (e.g., B cells, CD4+ cells, CD8+ cells, NK cells, etc.)

Feel free to inquire before ordering, or specify your interest in the “Order notes” section during checkout, and we will follow up with you shortly after your order is processed.