- High-quality PBMCs from multiple myeloma blood donors sourced from a world-renowned blood center
- High viability (> 90%) after thawing
- Used for a wide variety of immunology-based applications, including in vitro immunoassays, population characterization, sequencing studies, and biologic testing
- All orders come with an iQ Certificate of Analysis
- Normally ships out same business day
Human Multiple Myeloma (MM) Peripheral Blood Mononuclear Cells (PBMCs)
About Multiple Myeloma and Our Donors
Multiple myeloma (MM) is a blood cancer that is characterized by rapidly proliferating plasma B cells. Normal plasma B cells secrete antibodies in response to infections and/or foreign substances. However, in multiple myeloma, plasma B cells divide continuously, which interferes with the production and development of other blood cells in the bone marrow. Subsequent crowding of the bone marrow space will also lead to aberrant plasma cells exiting the space and causing further damage to outside organs.
Abnormal plasma cells also secrete a substance called monoclonal (M) protein that is found in the blood and urine. If in large amounts, kidney dysfunction may follow due to the inability to process it. Other symptoms of MM include fatigue, bone pain, fractures, and recurring infections.
Our MM cells, or PBMCs from MM patients, are sourced from a renowned world-class blood collection center that has been a pioneer in many screening processes. All MM patients have been tested for various infectious diseases, including HIV and Hepatitis B/C, as well as normal levels of hemoglobin. Further, donors undergo a screening questionnaire to disclose vital statistics, medication, cancer history, and travel history to ensure that their PBMCs will reliably serve your research endeavors.
Application Summary for Multiple Myeloma Cells
MM cells can be used for a wide variety of immunology-based applications, in particular ex-vivo applications that characterize immune cell populations based on immunophenotyping. MM cells are also an ideal source of target cells for cytotoxicity assays, including ADCC and CDC assays, in the presence of your therapeutic molecule.
Isolation of specific immune cell types from MM cells can also be performed to obtain an enriched population in order to carry out genome or transcriptome sequencing.
Isolation of Multiple Myeloma Cells
Peripheral blood is obtained from responsible facilities that have many years of experience collecting samples. Since the facilities are local, the blood is quickly transported to iQ Bioscience’s laboratory for processing.
Once at our lab, MM cells are isolated by performing density centrifugation with Ficoll, a high molecular weight sucrose solution, to remove red blood cells under sterile conditions. Depending on the species (human or non-human) of blood, either 1.077 or 1.084 g/ml Ficoll is used, resulting in a perfect layer of mononuclear cells that are separated from plasma, platelets, granulocytes, and erythrocytes. Additional quality control steps are taken to isolate and prepare PBMCs that ensure the highest viability for cryopreservation and downstream experimental applications.
Cryopreservation and Storage
Our MM cells are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 90%) after thawing.
Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).
Quality Control Process
We implement random sampling per lot to test for viability and cell counts to ensure they meet specifications, which are recorded on the Certificate of Analysis that is included with each shipment. Cell counts are obtained using a manual hemocytometer and then cross-referenced with an automated cell counter. Each lot is also characterized for unique cell populations by immunophenotyping, in which the results are also recorded on the Certificate of Analysis.
We are Committed to Ethical Practices
iQ Biosciences’ human primary cell products are lawfully obtained in accordance with Local, State, and Federal U.S. requirements, and the collection of cells complies with ethical requirements. Our cells are obtained from normal or disease patient volunteers participating in a donor program that is approved by an Institutional Review Board (IRB) or Human Subject Committee. A signed and witnessed consent form is obtained from donor volunteers prior to starting the collection protocol. Strict controls on personal identifiers of volunteers are in place in order to protect their privacy.
For US customers, we ship via FedEx Overnight Shipping. Shipping charges will vary per shipping address (based on ZIP code) and are estimated to be $110.
For international (non-US) customers, we work closely with you and our couriers to ensure all necessary documentation is in place for international shipments to significantly reduce the chance of delays at Customs. For the export of non-human primate samples, this includes preparing CITES permits, as well as any other documentation as required by country. Please submit an inquiry to firstname.lastname@example.org for your estimated time of delivery and shipping charges.
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