Purified Cynomolgus Monkey CD20+ B Cells

Description

About the Cynomolgus Monkey (or Cynomolgus Macaque)

The cynomolgus macaque (“cyno”) is the most commonly utilized non-human primate in biomedical research. According to some researchers, these monkeys are ideal models due to the 90-93% genetic similarity to and recent evolutionary divergence from humans. They are employed in numerous research areas, such as immunology, neuroscience, oncology, diabetes, and pharmacology due to their genetics and physiology.

Cynomolgus Macaque Monkey CD20⁺ B Cells

CD20⁺ B cells make up approximately 5-15% of PBMCs in adult cynomolgus monkeys. These B lymphocytes play a vital role in the adaptive immune system, particularly humoral immunity by producing antigen-specific immunoglobins (Ig) that neutralize pathogens and toxins and tag them for phagocytosis. Upon activation, B cells differentiate into plasma cells, which secrete large quantities of antibodies to bind antigens, promoting pathogen neutralization or clearance. Furthermore, B cells act as antigen-presenting cells, assisting other immune cells like T lymphocytes, and also contribute to the body’s inflammatory response, enhancing immune defense mechanisms.

Cyno CD20⁺ B Cell Application Summary

Cynomolgus CD20+ B cells are integral in numerous areas of biomedical research due to their roles in the immune response and antibody production. Examples include:

• Monoclonal antibodies – B cells are essential for the development of monoclonal antibodies, which are widely used therapeutics in treating various diseases. Additionally, B cells are studied to understand the pathogenesis of diseases, contributing to the identification of new therapeutic targets and improving treatment strategies.
• Cancer immunotherapy – researchers study B cells to enhance their anti-tumor immune responses, including how they can aid T cells in recognizing cancer cells.
• Autoimmune disease research – B cells are investigated to better understand mechanisms of autoimmunity, leading to targeted therapies that modulate B cell activity.
• Transplantation studies – B cells are examined to improve graft outcomes by controlling immune responses that may lead to graft rejection.
• Vaccine development – B cells are key for evaluating the immune response, helping to assess how vaccines stimulate antibody production and formation of immune memory which is important for predicting vaccine efficacy.
• Infectious disease research – B cells are utilized for their function in pathogen recognition, neutralization, and long-term immunity, aiding in the development of treatments and vaccines.
• Immunology research – B cells provide insights into overall immune system development and regulation.

Cyno CD20⁺ B Cell Purification

Collection of samples

Cynomolgus PBMCs were sourced from a responsible third-party vendor that operate according to local regulations and laws.

Magnetic Positive Isolation of CD20⁺ B Cells

To purify cynomolgus CD20⁺ B cells, PBMCs were incubated with antibodies against CD20⁺ and subsequently passed through a magnetic column. The cells that pass through are the non-CD20⁺ cells, which can be used for other purposes, while the labeled CD20⁺ B cells are left bound to the column. These cells are subsequently eluted to obtain purified CD20⁺ B cells.

Contact us for more information about purchasing our magnetically isolated CD20⁺ B cells.

Purity

A small aliquot of Cyno CD20⁺ B cells was tested for post-sort purity by flow cytometry analysis. Purity of CD20⁺ B cells, as defined by CD20⁺ expression, was > 94% (Figure 1) demonstrating that the purification method does not interfere with the detection method due to the abundance of marker on the cell surface.

Figure 1. Purity of CD20⁺ B cells after magnetic isolation from cynomolgus PBMCs. Cynomolgus PBMCs were incubated with antibody against CD20 and then subjected to magnetic separation to obtain CD20⁺ B cells. A small aliquot was taken after selection to evaluate post-sort purity by flow cytometry.

Cryopreservation and storage

Purified CD20⁺ B cells were cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 90%) after thawing.

Cells should be stored at < -120 °C once they are received, such as within a liquid nitrogen tank (vapor phase).