{"id":327,"date":"2016-10-01T12:01:03","date_gmt":"2016-10-01T12:01:03","guid":{"rendered":"http:\/\/iqbiosciences.com\/?post_type=product&p=327"},"modified":"2023-06-21T13:29:08","modified_gmt":"2023-06-21T20:29:08","slug":"cd1-mouse-splenocytes","status":"publish","type":"product","link":"https:\/\/iqbiosciences.com\/product\/cd1-mouse-splenocytes\/","title":{"rendered":"CD1 Mouse Splenocytes"},"content":{"rendered":"

About the CD1 Mouse<\/h3>\n

The CD1 mouse is a commonly-used outbred strain for general multipurpose studies, but has also been extensively studied in oncology, toxicology, aging, and immunology. Because they are outbred, the genetic profile of these mice is unknown. However, their immune cells (like these CD1 mouse splenocytes) are routinely used in immune-based assays when genetic information is not specified. This may offer benefits when comparing the immune response to that of cells from inbred mice.<\/p>\n

Application Summary for CD1 Mouse Splenocytes<\/h3>\n

CD1 mouse splenocytes can be used for a wide variety of immunology-based applications. In-vitro<\/em> immunoassays, such as ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to measure ADCC<\/a> or CDC<\/a>, and plaque assays are routinely performed using these cells. In addition, mouse splenocytes are also used in ex-vivo\u00a0<\/em>applications, such as cell population characterization and generation of genetically modified cells through retroviral infection.<\/p>\n

Isolation of Splenocytes<\/h3>\n

Spleen Collection and Splenocyte Isolation<\/h4>\n

Spleens are isolated responsibly from CD1 mice at third party facilities. These facilities are vetted to ensure they operate according to local regulations and laws. Since the facility is local, the tissue is quickly transported to iQ\u2019s facility for processing.<\/p>\n

Once at our facility, the splenic tissue is carefully dissociated through a gentle mechanical process to generate a single cell suspension under sterile conditions. The suspension is then carefully passed through a filter to remove any fat and\/or connective tissue and subsequently depleted of red blood cells in a manner that is not damaging to the other cells. The purified splenocytes are then frozen based on iQ Biosciences\u2019 quality-controlled cryopreservation protocol to promote recoverability and viability once in the end user\u2019s hands.<\/p>\n

Cryopreservation and Storage<\/h3>\n

Our CD1 mouse splenocytes are cryopreserved carefully using iQ Biosciences\u2019 cryopreservation protocol that ensures high viability (typically > 70%) after thawing.<\/p>\n

Cells should be stored at < -120\u00b0C once they are received, such as within a liquid nitrogen tank (vapor phase).<\/p>\n","protected":false},"excerpt":{"rendered":"