{"id":328,"date":"2016-10-01T12:02:13","date_gmt":"2016-10-01T12:02:13","guid":{"rendered":"http:\/\/iqbiosciences.com\/?post_type=product&p=328"},"modified":"2024-03-12T16:52:38","modified_gmt":"2024-03-12T23:52:38","slug":"wistar-rat-splenocytes","status":"publish","type":"product","link":"https:\/\/iqbiosciences.com\/product\/wistar-rat-splenocytes\/","title":{"rendered":"Wistar Rat Splenocytes"},"content":{"rendered":"

About the Wistar Rat<\/h3>\n

The Wistar rat is one of the two most routinely-used albino rat strains in biomedical research. They are commonly used in many fields of research, including oncology, aging, pharmacology, toxicology, and metabolism\/nutrition. They are also used in immunology as models for infectious diseases and autoimmune responses. Depending on the parameters of your experiments, cells from Wistar rats, like Wistar rat splenocytes, may offer experimental advantages.<\/p>\n

Application Summary for Wistar Rat Splenocytes<\/h3>\n

Wistar rat splenocytes can be used for a wide variety of immunology-based applications. In-vitro<\/em> immunoassays, such as ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to measure ADCC<\/a> or CDC<\/a>, and plaque assays are routinely performed using these cells. In addition, rat splenocytes are also used in ex-vivo\u00a0<\/em>applications, such as cell population characterization and generation of genetically modified cells through retroviral infection.<\/p>\n

Isolation of CD45.1 Splenocytes<\/h3>\n

Spleen Collection and Splenocyte Isolation<\/h4>\n

Spleens are isolated responsibly from Wistar rats at third party facilities. These facilities are vetted to ensure they operate according to local regulations and laws. Since the facility is local, the tissue is quickly transported to iQ\u2019s facility for processing.<\/p>\n

Once at our facility, the splenic tissue is carefully dissociated through a gentle mechanical process to generate a single cell suspension under sterile conditions. The suspension is then carefully passed through a filter to remove any fat and\/or connective tissue and subsequently depleted of red blood cells in a manner that is not damaging to the other cells. The purified splenocytes are then frozen based on iQ Biosciences\u2019 quality-controlled cryopreservation protocol to promote recoverability and viability once in the end user\u2019s hands.<\/p>\n

Cryopreservation and Storage<\/h3>\n

Our Wistar rat splenocytes are cryopreserved carefully using iQ Biosciences\u2019 cryopreservation protocol that ensures high viability (typically > 70%) after thawing.<\/p>\n

Cells should be stored at < -120\u00b0C once they are received, such as within a liquid nitrogen tank (vapor phase).<\/p>\n","protected":false},"excerpt":{"rendered":"