Description

About the BALB/c Mouse

The BALB/c mouse is a commonly-used inbred mouse strain for the study of autoimmune diseases and pathogen-driven immune response. They carry the H-2^d haplotype and their immune cells are routinely used in immune-based assays. Based on early studies, the BALB/c mouse is biased towards a Th2 antibody-mediated immune response when challenged appropriately. Thus, the BALB/c mouse is well-studied in the field of immunology and their cells (like these BALB/c Mouse PBMCs) may offer experimental advantages depending on the parameters of your experiments.

Application Summary for BALB/c Mouse PBMCs

BALB/c Mouse PBMCs can be used for a wide variety of immunology-based applications. In-vitro immunoassays, such as ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to measure ADCC or CDC, and plaque assays are routinely performed using these cells. In addition, PBMCs are also used in ex-vivo applications, such as cell population characterization and generation of genetically modified cells through retroviral infection.

Isolation of PBMCs

Peripheral blood is obtained from responsible facilities that have many years of experience collecting samples. Since the facilities are local, the blood is quickly transported to iQ Bioscience’s laboratory for processing.

Once at our lab, PBMCs are isolated by performing density centrifugation with Ficoll, a high molecular weight sucrose solution, to remove red blood cells under sterile conditions. Depending on the species (human or non-human) of blood, either 1.077 or 1.084 g/ml Ficoll is used, resulting in a perfect layer of mononuclear cells that are separated from plasma, platelets, granulocytes, and erythrocytes. Additional quality control steps are taken to isolate and prepare PBMCs that ensure the highest viability for cryopreservation and downstream experimental applications.

Cryopreservation and Storage

Our mouse PBMCs are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 80%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).

Immunophenotyping Data for BALB/c Mouse PBMCs

To provide deeper insights into the cellular composition of our BALB/c Mouse PBMCs, we have characterized the distribution of key immune cell populations. Below is a representative profile of cell types based on historical data:

*These percentages are for 6 to 10 week old mice. These values may differ depending on factors such as mouse age, gender, and environmental factors. Data is sourced from internal immunophenotyping analysis. Values are expressed as % of total leukocytes in each sample, unless otherwise specified.