Description

About the CD1 Mouse

The CD1 mouse is a commonly-used outbred strain for general multipurpose studies, but has also been extensively studied in oncology, toxicology, aging, and immunology.  Because they are outbred, the genetic profile of these mice is unknown.  However, their immune cells (like these CD1 mouse PBMCs) are routinely used in immune-based assays when genetic information is not specified. This may offer benefits when comparing the immune response to that of cells from inbred mice.

Application Summary for CD1 Mouse PBMCs

PBMCs can be used for a wide variety of immunology-based applications. In-vitro immunoassays, such as ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to measure ADCC or CDC, and plaque assays are routinely performed using these cells. In addition, PBMCs are also used in ex-vivo applications, such as cell population characterization and generation of genetically modified cells through retroviral infection.

Isolation of PBMCs

Peripheral blood is obtained from responsible facilities that have many years of experience collecting samples. Since the facilities are local, the blood is quickly transported to iQ Bioscience’s laboratory for processing.

Once at our lab, PBMCs are isolated by performing density centrifugation with Ficoll, a high molecular weight sucrose solution, to remove red blood cells under sterile conditions. Depending on the species (human or non-human) of blood, either 1.077 or 1.084 g/ml Ficoll is used, resulting in a perfect layer of mononuclear cells that are separated from plasma, platelets, granulocytes, and erythrocytes. Additional quality control steps are taken to isolate and prepare PBMCs that ensure the highest viability for cryopreservation and downstream experimental applications.

Cryopreservation and Storage

Our mouse PBMCs are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 80%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).