CD1 Mouse Peripheral Blood Mononuclear Cells (PBMCs)

CD1 Mouse Peripheral Blood Mononuclear Cells (PBMCs)

$600.00

  • High-quality PBMCs from the CD1 mouse, a commonly-used outbred mouse strain
  • High viability (> 80%) after thawing
  • Used for a wide variety of immunology-based applications, including in-vitro immunoassays and ex-vivo applications
  • All orders come with an iQ Certificate of Analysis
  • Normally ships out same business day
SKU: IQB-MPB3xx Categories: , , Tags: , ,

Description

About the CD1 Mouse

The CD1 mouse is a commonly-used outbred strain for general multipurpose studies, but has also been extensively studied in oncology, toxicology, aging, and immunology.  Because they are outbred, the genetic profile of these mice is unknown.  However, their immune cells (like these CD1 mouse PBMCs) are routinely used in immune-based assays when genetic information is not specified. This may offer benefits when comparing the immune response to that of cells from inbred mice.

Application Summary for CD1 Mouse PBMCs

PBMCs can be used for a wide variety of immunology-based applications. In-vitro immunoassays, such as ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to measure ADCC or CDC, and plaque assays are routinely performed using these cells. In addition, PBMCs are also used in ex-vivo applications, such as cell population characterization and generation of genetically modified cells through retroviral infection.

Isolation of PBMCs

Peripheral blood is obtained from responsible facilities that have many years of experience collecting samples. Since the facilities are local, the blood is quickly transported to iQ Bioscience’s laboratory for processing.

Once at our lab, PBMCs are isolated by performing density centrifugation with Ficoll, a high molecular weight sucrose solution, to remove red blood cells under sterile conditions. Depending on the species (human or non-human) of blood, either 1.077 or 1.084 g/ml Ficoll is used, resulting in a perfect layer of mononuclear cells that are separated from plasma, platelets, granulocytes, and erythrocytes. Additional quality control steps are taken to isolate and prepare PBMCs that ensure the highest viability for cryopreservation and downstream experimental applications.

Cryopreservation and Storage

Our mouse PBMCs are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 80%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).

Additional information

Available Size(s)

5 million cells / vial

Format

Frozen

Cell Type

Mononuclear

Tissue Type

Normal Peripheral Blood

Species

Mouse

Viability

> 80%

Request a Cellular Products Fact Sheet for Non-Human PBMCs

Interested in learning more about this Cellular Product? Complete the form below to request a Fact Sheet.






Tailored Experiments

Your research is important to us. Our scientists will work through each step of the process with you, including assay design, data analysis, and recommendations for future studies.

Streamlined Process

Simplify your workflow. Bypass the middle-man: at iQ Biosciences, you'll get immediate access to our biospecimen inventory, saving you both valuable time and money.

Expertise

We're experts- so you don't have to be. Augmenting years of experience in immunology and working with immune assays, our scientists stay current with the latest publications and technology.

Exceptional Service

We're here to help. We know the challenges you're facing: whether it be through expedited service or our complimentary consulting services, our team is dedicated to helping you reach your goals.

 

For US customers, we ship via FedEx Overnight Shipping. Shipping charges will vary per shipping address (based on ZIP code) and are estimated to be $110.

For international (non-US) customers, we work closely with you and our couriers to ensure all necessary documentation is in place for international shipments to significantly reduce the chance of delays at Customs. For the export of non-human primate samples, this includes preparing CITES permits, as well as any other documentation as required by country. Please submit an inquiry to orders@iqbiosciences.com for your estimated time of delivery and shipping charges.

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