Purified Cynomolgus Macaque Monkey CD8 T Cells

Purified Cynomolgus Macaque Monkey CD8 T Cells

$950.00

  • CD8 T cells purified from cynomolgus macaque PBMCs by negative selection
  • Used for a wide variety of safety assessment and functional assays
  • Carefully cryopreserved to ensure high viability upon thawing
  • All orders come with an iQ Certificate of Analysis
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Description

About the Cynomolgus Macaque Monkey

The cynomolgus macaque is the most commonly used non-human primate used in biomedical research.  According to some researchers, these monkeys are ideal models due to the 90-93% genetic similarity to and recent evolutionary divergence from humans.  They are employed in numerous research areas, such as immunology, neuroscience, oncology, diabetes, and pharmacology due to their genetics and physiology.

Cynomolgus Macaque Monkey CD8 T Cells

Cynomolgus CD8 T cells make up approximately 8-28% of PBMCs in a typical adult monkey. These cells carry the CD8 protein marker and the T cell receptor (TCR), which recognizes foreign antigens presented by major histocompatibility complex class I molecules on antigen presenting cells to activate them.

CD8 T cells develop in the thymus to become mature T cells and exit the thymus to reside and encounter foreign pathogens in lymphoid tissues.

When activated, CD8 T cells become cytotoxic cells that can lyse infected or transformed cells.

CD8 T Cell Application Summary

Purified cynomolgus CD8 T cells are a good source of cells to study the biology of CD8 T cells and their role in the immune system.  They can be used for a variety of stimulation-dependent functional experiments to assess cytotoxicity and proliferation.

With the current trend toward development of immuno-modulatory drugs, purified cynomolgus CD8 T cells are an ideal source of cells to test cytotoxic T cell response in the presence of therapeutic molecules.  More importantly, cynomolgus T cells can be used to assess toxicity and safety characteristics of these biologics in the presence of CD8 T cell activation before first in-human trials.  These cells are commonly employed in pre-clinical settings to ensure biologics are not eliciting unwanted functions, specifically from CD8 T cells.

The negative isolation of CD8 T cells leaves them untouched without any antibody binding to cell surface markers that may influence function. This method leaves all cell surface proteins eligible to be bound to antibodies or other molecules for functional or population characterization studies.

CD8 T Cell Purification

Collection of samples

Cynomolgus PBMCs were sourced from a responsible third party vendor that operate according to local regulations and laws.

Negative Isolation of CD8 T cells

To purify cynomolgus CD8 T cells, PBMCs were incubated with antibodies against CD4 T cells, B cells, NK cells, dendritic cells, monocytes, granulocytes, and erythrocytes, and subsequently run through a magnetic column. Cells labeled with the antibodies were bound to the column, while unlabeled cells, the CD8 T cells, passed through.

Purity

A small aliquot of cells was tested for post-sort purity by flow cytometry analysis. Purity of CD8 T cells, as defined by CD8 expression, was > 94% (Figure 1).

Cyno CD8 T Cells Before and After Purification

Figure 1. Purity of CD8 T cells after selection by negative isolation using magnetic column separation from PBMCs.  In this representation, cynomolgus PBMCs were incubated with antibodies against CD4 T cells, B cells, NK cells, dendritic cells, monocytes, granulocytes, and erythrocytes and passed through a magnetic column to obtain CD8 T cells by negative selection.  A small aliquot was taken after selection to evaluate post-sort purity (right).  Percent of CD8+ cells in PBMCs is also shown (left).

Cryopreservation and storage

Purified CD8 T cells were cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability after thawing.

Cells should be stored at <-120°C once they are received, such as within a liquid nitrogen tank (vapor phase).

Additional information

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Please let us know in the notes section during checkout if you are interested in animal donor-matched PBMCs, as we carry these in stock.

If you would like to request multiple animal donors or the same animal donor, please specify this in the notes section as well.

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