Description

About the Boxer Canine

The Boxer canine is a commonly-used breed of dog in research, which is prone to a number of genetically-driven health problems, such as a high incidence of brain and central nervous system cancers. In addition, they have been used to study a number of lymphomas and gastrointestinal diseases. Because many of these issues are genetically driven, extensive sequencing has been performed on various boxer canine cell and cancer types.

Boxers are also used in toxicity studies for a variety of therapeutics developed by pharmaceutical and biotech companies.

Application Summary for Boxer Canine PBMCs

Boxer canine PBMCs can be used for a wide variety of immunology-based applications, in particular ex-vivo applications that characterize immune cell populations based on immunophenotyping. In-vitro immunoassays, such as proliferation and cytotoxicity assays, can also be performed using these cells. Additionally, boxer canine PBMCs are a good source of cells to test cross-reactivity of molecules across a range of animals.

Isolation of specific immune cell types can also be performed to obtain an enriched population in order to carry out genome or transcriptome sequencing.

Isolation of PBMCs

Peripheral blood is obtained from responsible facilities that have many years of experience collecting samples. Since the facilities are local, the blood is quickly transported to iQ Bioscience’s laboratory for processing.

Once at our lab, PBMCs are isolated by performing density centrifugation with Ficoll, a high molecular weight sucrose solution, to remove red blood cells under sterile conditions. Depending on the species (human or non-human) of blood, either 1.077 or 1.084 g/ml Ficoll is used, resulting in a perfect layer of mononuclear cells that are separated from plasma, platelets, granulocytes, and erythrocytes. Additional quality control steps are taken to isolate and prepare PBMCs that ensure the highest viability for cryopreservation and downstream experimental applications.

Cryopreservation and Storage

Our canine PBMCs are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 80%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).