Description

About the New Zealand White Rabbit

The New Zealand White Rabbit is arguably the most widely-used outbred rabbit strain in biomedical research. They are commonly used in many fields of research, including dermatology, toxicology, ophthalmology, cardiovascular studies, and antibody production. Because the New Zealand White Rabbit offers an immune response to many pathogens similar to that found in humans, they are also used in immunology research as models for infectious diseases and autoimmune responses. Depending on the parameters of your experiments, cells from New Zealand White Rabbits like rabbit splenocytes, may offer experimental advantages.

Application Summary for New Zealand White Rabbit Splenocytes

New Zealand White Rabbit splenocytes can be used for a wide variety of immunology-based applications. In-vitro immunoassays, such as ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to measure ADCC or CDC, and plaque assays are routinely performed using these cells. In addition, rabbit splenocytes are also used in ex vivo applications, such as cell population characterization and generation of genetically modified cells through retroviral infection.

Isolation of New Zealand White Rabbit Splenocytes

Spleen Collection and Splenocyte Isolation

Spleens are isolated responsibly from NZW rabbits at third party facilities. These facilities are vetted to ensure they operate according to local regulations and laws. Since the facility is local, the tissue is quickly transported to iQ’s facility for processing.

Once at our facility, the splenic tissue is carefully dissociated through a gentle mechanical process to generate a single cell suspension under sterile conditions. The suspension is then carefully passed through a filter to remove any fat and/or connective tissue and subsequently depleted of red blood cells in a manner that is not damaging to the other cells. The purified splenocytes are then frozen based on iQ Biosciences’ quality-controlled cryopreservation protocol to promote recoverability and viability once in the end user’s hands.

Cryopreservation and Storage

Our NZW rabbit splenocytes are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (typically > 70%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).