CD45.1 C57BL/6 Mouse Peripheral Blood Mononuclear Cells (PBMCs)

CD45.1 C57BL/6 Mouse Peripheral Blood Mononuclear Cells (PBMCs)

$700.00

  • High-quality CD45.1 Peripheral Blood Mononuclear cells (PBMCs) from C57BL/6 congenic mice
  • High viability (> 80%) after thawing
  • Used for a wide variety of immunology-based co-culture applications or in vivo adoptive transfer studies
  • All orders come with an iQ Certificate of Analysis
  • Normally ships out same business day
SKU: IQB-MPB6xx Categories: , , Tags: , , ,

Description

CD45.1 C57BL/6 Peripheral Blood Mononuclear cells (PBMCs)

CD45.1 PBMCs come from C57BL/6 congenic mice that express the CD45.1 allele.  CD45 itself is a protein tyrosine phosphatase receptor expressed on all hematopoietic cells except mature erythrocytes and platelets and is encoded by the Ptprc gene.  Wild-type C57BL/6 mice normally carry the Ptprcb (CD45.2) allele while the other (congenic) strain carries the Ptprca (CD45.1) allele on the same C57BL/6 genetic background.

Application Summary for CD45.1 and CD45.2 Cells

CD45.1 and CD45.2 cells are widely used in transplant and adoptive transfer studies to track the donor and host cells through the different alleles.  In most transfer experiments, leukocytes expressing CD45.1 are transferred into wild-type C57BL/6 mice and then identified using an antibody specific for CD45.1.   Similarly, cells expressing CD45.2 can be distinguished using antibodies that recognize CD45.2.

Cells from congenic mice that are genetically engineered (knockout or transgenic mice) can also be used in in vitro experiments to track cell development.  Cells from knockout mice on a CD45.1 background can be mixed in culture with CD45.2 wild-type cells to understand the role of that specific gene in cell lineage fate decisions in a wild-type environment.  Similar to the animal studies, antibodies against CD45.1 and CD45.2 can be used to distinguish the origin of the cells and track their outcomes.  These experiments are faster and easier to perform and analyze compared to animal transfer studies.

Isolation of CD45.1 PBMCs

Peripheral blood collection and PBMC Isolation

PBMCs from non-human organisms are isolated from peripheral blood collected responsibly at third party facilities.  These facilities are vetted to ensure they operate according to local regulations and laws.  Since the facility is local, the blood is quickly transported to iQ’s facility for processing.

PBMCs are specifically isolated by performing a density centrifugation at room temperature to remove red blood cells.  iQ Biosciences performs this process under sterile conditions by using Ficoll, a high molecular weight sucrose solution from GE Healthcare.  The result is a perfect layer of mononuclear cells that are separated from plasma, platelets, granulocytes, and erythrocytes.  Additional quality control steps are taken to isolate and prepare PBMCs that ensure the highest viability for cryopreservation and downstream experimental applications.

Expression of CD45.1

A small aliquot of cells was tested for CD45.1 by flow cytometric analysis. Expression of CD45.1 was > 99%.

Cryopreservation and storage

CD45.1 PBMCs were cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 80%) after thawing.

Cells should be stored at <-120°C once they are received, such as within a liquid nitrogen tank (vapor phase).

Additional information

Available Size(s)

Cell Type

Format

Species

Tissue Type

Viability

> 80%

Tailored Experiments

Your research is important to us. Our scientists will work through each step of the process with you, including assay design, data analysis, and recommendations for future studies.

Streamlined Process

Simplify your workflow. Bypass the middle-man: at iQ Biosciences, you'll get immediate access to our biospecimen inventory, saving you both valuable time and money.

Expertise

We're experts- so you don't have to be. Augmenting years of experience in immunology and working with immune assays, our scientists stay current with the latest publications and technology.

Exceptional Service

We're here to help. We know the challenges you're facing: whether it be through expedited service or our complimentary consulting services, our team is dedicated to helping you reach your goals.

 

For US customers, we ship via FedEx Overnight Shipping. Shipping charges will vary per shipping address (based on ZIP code) and are estimated to be $110.

For international (non-US) customers, we work closely with you and our couriers to ensure all necessary documentation is in place for international shipments to significantly reduce the chance of delays at Customs. For the export of non-human primate samples, this includes preparing CITES permits, as well as any other documentation as required by country. Please submit an inquiry to orders@iqbiosciences.com for your estimated time of delivery and shipping charges.

Fisher Scientific (United States)
Tel: 1-800-766-7000
Web: https://www.fishersci.com

Quartzy (United States)
Web: https://www.quartzy.com

VWR International (United States)
Tel: 1-800-932-5000
Web: https://www.vwr.com

Cosmo Bio Co., Ltd (Japan)
Tel: 03-5632-9610
Fax: 03-5632-9619
Email: nsmail@cosmobio.co.jp
Web: https://www.cosmobio.co.jp

BioClone (South Korea)
Tel: +82-2-2690-0058
Email: bioclone@bioclone.co.kr
Web: http://www.bioclone.co.kr