Purified Rhesus Macaque Monkey NK Cells

Purified Rhesus Macaque Monkey NK Cells

$1,800.00

  • NK cells purified from Rhesus macaque PBMCs by magnetic selection
  • Used for a wide variety of safety assessment and functional assays
  • Carefully cryopreserved to ensure high viability (> 90%) upon thawing
  • All orders come with an iQ Certificate of Analysis

 

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Description

About the Rhesus Monkey (or Rhesus Macaque)

The rhesus macaque is one of the most commonly utilized non-human primates in biomedical research.  According to some researchers, these monkeys are ideal models due to the 90-93% genetic similarity to and recent evolutionary divergence from humans.  They are employed in numerous research areas, such as immunology, neuroscience, oncology, diabetes, and pharmacology due to their genetics and physiology.

Rhesus Macaque Monkey NK Cells

Rhesus NK cells make up approximately 2-18% of PBMCs in a typical adult monkey. These cells carry activating and inhibitory molecules that influence their activation status.

When activated, NK cells can lyse infected or transformed cells through natural cytotoxicity and antibody-dependent cellular cytotoxicity (ADCC).

NK Cell Application Summary

Purified Rhesus NK cells are a good source of cells to study the biology of NK cells and their role in the immune system.  They can be used for a variety of stimulation-dependent functional experiments to assess cytokine production and cytotoxicity.

With the current trend toward development of immuno-modulatory drugs, NK cells are an ideal source of cells to test cytotoxicity and cytokine production in the presence of therapeutic molecules.  More importantly, NK cells can be used to assess toxicity and safety characteristics of these biologics in the presence of NK cell activation before first in-human trials.  These cells are commonly employed in pre-clinical settings to ensure biologics are not eliciting unwanted functions, specifically from NK cells.

The positive isolation method of NK cells may lead to internalization of the marker that was used to isolate the cells. In most cases, these markers are only used for identification purposes and may not have any effect on function, but it will depend on the organism and function. Therefore, cells isolated using this method may be employed for functional or population characterization studies with the knowledge that the isolation marker may be internalized and not present.

NK Cell Purification

Collection of samples

Rhesus PBMCs were sourced from a responsible third party vendor that operate according to local regulations and laws.

Magnetic Positive Isolation of Rhesus NK cells

To purify rhesus NK cells, PBMCs were incubated with antibodies against an NK cell-specific marker, which will vary depending on the species of the organism, and subsequently passed through a magnetic column. The cells that pass through are the non-NK cells, which can be used for other purposes, while the labeled NK cells are left bound to the column. These cells are subsequently eluted to obtain purified NK cells.

Cryopreservation and storage

Purified NK cells were cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 90%) after thawing.

Cells should be stored at < -120 °C once they are received, such as within a liquid nitrogen tank (vapor phase).

Additional information

Available Size(s)

Cell Type

Format

Species

Tissue Type

Viability

> 90%

Please let us know in the notes section during checkout if you are interested in animal donor-matched PBMCs, as we carry these in stock.

If you would like to request multiple animal donors or the same animal donor, please specify this in the notes section as well.

Request a Cellular Products Fact Sheet for Non-Human PBMCs

Interested in learning more about this Cellular Product? Complete the form below to request a Fact Sheet.





Tailored Experiments

Your research is important to us. Our scientists will work through each step of the process with you, including assay design, data analysis, and recommendations for future studies.

Streamlined Process

Simplify your workflow. Bypass the middle-man: at iQ Biosciences, you'll get immediate access to our biospecimen inventory, saving you both valuable time and money.

Expertise

We're experts- so you don't have to be. Augmenting years of experience in immunology and working with immune assays, our scientists stay current with the latest publications and technology.

Exceptional Service

We're here to help. We know the challenges you're facing: whether it be through expedited service or our complimentary consulting services, our team is dedicated to helping you reach your goals.

 

For US customers, we ship via FedEx Overnight Shipping. Shipping charges will vary per shipping address (based on ZIP code) and are estimated to be $110.

For international (non-US) customers, we work closely with you and our couriers to ensure all necessary documentation is in place for international shipments to significantly reduce the chance of delays at Customs. For the export of non-human primate samples, this includes preparing CITES permits, as well as any other documentation as required by country. Please submit an inquiry to orders@iqbiosciences.com for your estimated time of delivery and shipping charges.

Fisher Scientific (United States)
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