Purified Cynomolgus Monkey CD3 Pan T Cells

Purified Cynomolgus Monkey CD3 Pan T Cells

$850.00$1,500.00

  • CD3 Pan T cells purified from cynomolgus macaque PBMCs by magnetic selection
  • Used for a wide variety of safety assessment and functional assays
  • Carefully cryopreserved to ensure high viability (> 90%) upon thawing
  • All orders come with an iQ Certificate of Analysis
SKU: IQB-Mn1-Txx Categories: , , Tags: , , , , , ,

Description

About the Cynomolgus Monkey (or Cynomolgus Macaque)

The cynomolgus macaque is the most commonly utilized non-human primate in biomedical research.  According to some researchers, these monkeys are ideal models due to the 90-93% genetic similarity to and recent evolutionary divergence from humans.  They are employed in numerous research areas, such as immunology, neuroscience, oncology, diabetes, and pharmacology due to their genetics and physiology, which is why Cyno CD3 Pan T cells are often used.

Cyno CD3 Pan T Cells

Cynomolgus monkey CD3 pan T cells (or simply Cyno CD3 pan T cells) consist of both CD4 and CD8 T cells, and make up approximately 52-80% of PBMCs in a typical adult monkey.  All T cells carry the CD3 protein marker and the T cell receptor (TCR), which recognizes foreign antigens presented by major histocompatibility complex molecules on antigen presenting cells to activate them.

T cells develop in the thymus to become mature CD4 or CD8 T cells and exit the thymus to reside and encounter foreign pathogens in lymphoid tissues.

When activated, CD4 T cells will become T helper subsets to secrete cytokines to boost either the humoral or cell-mediated immune response. In contrast, CD8 T cells, when stimulated, will become cytotoxic T cells that can lyse infected or transformed cells.  Together, these two main subsets work to clear infections or diseased cells.

Cyno CD3 Pan T Cell Application Summary

Purified cynomolgus T cells (or simply Cyno CD3 pan T cells) are a good source of cells to study the biology of T cells and their role in the immune system. T cells are responsible for producing cytokines and performing cytotoxicity.  Thus, pan T cells can be used for a variety of stimulation-dependent functional experiments to assess cytokine production, proliferation, and cytotoxicity.

With the current trend toward development of immuno-modulatory drugs, purified cynomolgus pan T cells are an ideal source of cells to test T cell response in the presence of therapeutic molecules.  More importantly, cynomolgus T cells can be used to assess toxicity and safety characteristics of these biologics in the presence of T cell activation before first in-human trials.  These cells are commonly employed in pre-clinical settings to ensure biologics are not eliciting unwanted functions, such as cytokine release and toxicity, specifically from T cells.

The negative isolation of pan T cells leaves them untouched without any antibody binding to cell surface markers that may influence function. This method leaves all cell surface proteins eligible to be bound to antibodies or other molecules for functional or population characterization studies.

In contrast, positive isolation of pan T cells may lead to internalization of the marker that was used to isolate the cells.  In most cases, these markers are only used for identification purposes and may not have any effect on function, but it will depend on the organism and function.  Therefore, cells isolated using this method may also be employed for functional or population characterization studies with the knowledge that the isolation marker may be internalized and not present.

Cyno CD3 Pan T Cell Purification

Collection of samples

Cynomolgus PBMCs were sourced from a responsible third party vendor that operate according to local regulations and laws.

Isolation of Cyno CD3 Pan T cells

To enrich for cyno CD3 pan T cells by the negative isolation method, PBMCs were incubated with antibodies against B cells, NK cells, dendritic cells, monocytes, granulocytes, and erythrocytes in a test tube, and subsequently subjected to a magnet. Cells labeled with the antibodies bound to the magnet through the test tube wall, while unlabeled cells, the CD3 pan T cells, were decanted into a fresh tube to obtain the enriched population.

For the positive selection method, PBMCs were incubated in a test tube with antibodies against a pan T cell-specific marker and subsequently subjected to a magnet, similar to negative isolation.  However, the cells that were decanted into the fresh test tube were the non-pan T cells, which can be used for other purposes, while the labeled pan T cells were left bound to the magnet through the test tube wall.  The test tube was then removed from the magnet to release the purified pan T cells directly into the test tube.

Contact us for more information about purchasing our magnetically isolated pan T cells.

Purity

A small aliquot of Cyno CD3 pan T cells was tested for post-sort purity by flow cytometry analysis. Purity of pan-T cells, as defined by CD3 expression, was > 97% (Figure 1).

Cyno CD3 Pan T Cells Before and After Purification

Figure 1. Purity of Cyno CD3 Pan T cells after negative magnetic isolation from PBMCs.  In this representation, cynomolgus PBMCs were incubated with antibodies against B cells, NK cells, dendritic cells, monocytes, granulocytes, and erythrocytes and subjected to negative magnetic isolation to obtain untouched pan-T cells.  A small aliquot was taken after selection to evaluate post-sort purity (right).  Percent of CD3+ cells in PBMCs is also shown (left).

Cryopreservation and storage

Purified CD3 pan T cells were cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 90%) after thawing.

Cells should be stored at < -120 °C once they are received, such as within a liquid nitrogen tank (vapor phase).

Additional information

Available Size(s)

,

Cell Type

Format

Species

Tissue Type

Viability

> 90%

Please let us know in the notes section during checkout if you are interested in animal donor-matched PBMCs, as we carry these in stock.

If you would like to request multiple animal donors or the same animal donor, please specify this in the notes section as well.

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We are Committed to Ethical Practices

iQ Biosciences’ primary cell products from non-human primates are collected under approved IACUC protocol, which was developed in consultation with the Attending Veterinarian and is consistent with current veterinary standards. The collection facility is AAALACi and PHS accredited, and all animal housing, handling, and research protocols are consistent with standards set forth by the Animal Welfare Act, NIH's Policy on Humane Care and Use of Animals and the Guide for the Care and Use of Animals. All animal housing and handling is done in such a way to minimize stress and risk of injury to the animal.

Tailored Experiments

Your research is important to us. Our scientists will work through each step of the process with you, including assay design, data analysis, and recommendations for future studies.

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For US customers, we ship via FedEx Overnight Shipping. Shipping charges will vary per shipping address (based on ZIP code) and are estimated to be $110.

For international (non-US) customers, we work closely with you and our couriers to ensure all necessary documentation is in place for international shipments to significantly reduce the chance of delays at Customs. For the export of non-human primate samples, this includes preparing CITES permits, as well as any other documentation as required by country. Please submit an inquiry to orders@iqbiosciences.com for your estimated time of delivery and shipping charges.

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