- Human monocytes are purified from PBMCs of healthy blood donors sourced from a world-renowned blood center
- Greater than 90% post-selection purity achieved via negative isolation using magnetic columns
- Carefully cryopreserved to ensure high viability upon thawing
- All orders come with an iQ Certificate of Analysis
Purified Human Monocytes
Human Monocytes Application Summary
Purified blood human monocytes are a good source of cells to study the role of monocytes, macrophages, and dendritic cells in the immune response. This is because blood monocytes are commonly used to differentiate into macrophages or dendritic cells with the appropriate cytokine environment. Together, these 3 cell types of the innate immune system serve very important functions, including phagocytosis, cytokine production to enhance the immune response, and antigen presentation to T cells.
The negative isolation of monocytes leaves them untouched without any antibody binding to cell surface markers. This leaves all cell surface proteins eligible to be bound to antibodies or other molecules for functional or population characterization studies.
Human Monocyte Enrichment
Collection of samples
Leukapheresis was performed on verified healthy donors at a world-renowned blood center to obtain PBMCs. PBMCs were carefully washed to remove excess buffers according to iQ Biosciences’ standard operating procedures to obtain clean and healthy PBMCs.
Negative isolation of monocytes
To enrich for monocytes, PBMCs were incubated with antibodies against T cells, B cells, NK cells, dendritic cells, granulocytes, and erythrocytes, and subsequently run through a magnetic column. Cells labeled with the antibodies were bound to the column, while unlabeled cells, the monocytes, passed through.
Following enrichment, a small aliquot of cells was taken to test for post-sort purity by flow cytometry analysis. Purity of monocytes, as defined by CD14 expression, was >94% (Figure 1).
Figure 1. Purity of monocytes after negative isolation by magnetic column separation from PBMCs. Human PBMCs were incubated with antibodies against T cells, B cells, NK cells, dendritic cells, granulocytes, and erythrocytes, and passed through a magnetic column to obtain untouched monocytes. A small aliquot was taken after selection to evaluate post-sort purity (right). Percent of CD14+ cells in PBMCs is also shown (left).
Cryopreservation and storage
Purified monocytes were cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability after thawing.
Cells should be stored at <-120°C once they are received, such as within a liquid nitrogen tank (vapor phase).
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