Expanded Human CD3 T Cells

Expanded Human CD3 T Cells

$350.00$1,210.00

  • CD3+ T cells expanded from purified human T cells
  • Expanded T cells are validated for retention of functional activity such as proliferation, cytokine production, and cytolytic activity
  • Applications include a wide variety of safety assessment and functional assays
  • Could be used for in vivo studies
  • Carefully cryopreserved to ensure high viability (> 90%) upon thawing
  • All orders come with an iQ Certificate of Analysis
SKU: IQB-Hu1-TxxE Categories: , , Tags: , , , ,

Description

Human CD3+ T cell Potential Application Summary

  • Flow cytometry-based binding studies for target binding and cross-reactivity
  • Large-scale potency assays to compare a panel of test articles for the ability to elicit T cell responses
  • Adoptive transfer into immunodeficient recipient mice to support the in vivo evaluation of activity with therapeutic test articles

Expansion of Human CD3+ T cells

These human CD3+ T cells are meticulously expanded over a multi-day period using a combination of cytokines, expansion medium, and high quality T cell activation beads. The expansion process takes place in a controlled environment to ensure optimal conditions for cell growth and retained function.

CD3+ purity is maintained after expansion.

(a)

CD4+ T cell frequency decreases after expansion with a concordant increase in CD8+ T cell frequency.

(b)

Figure 1: T cell phenotype is maintained post-expansion. (a) CD3+ purity is maintained after expansion. (b) CD4+ T cell frequency decreases after expansion with a concordant increase in CD8+ T cell frequency. Data shows one representative sample.

Validation of Expanded Human CD3+ T cells

Our expanded human CD3+ T cells have undergone rigorous testing to validate their exceptional quality and functional ability as reflected by their capacity for robust proliferation, target cell killing, and cytokine production, in vitro. These cells may be used for various applications, including mechanistic research, therapeutic development, and innovative cellular assays.

Proliferation

CD8+ T cells within expanded human CD3+ T cells proliferate in response to stimulation

Figure 2: CD8+ T cells within expanded human CD3+ T cells proliferate in response to stimulation. Expanded human T cells were stimulated with activation beads for 96 hours. Flow cytometry was used to assess the frequency of Ki-67+ (a marker of proliferation) cells within CD8+ T cells. Data shows the average (mean) of technical triplicates with SEM error bars.

Cytokine Release

Expanded human CD3+ T cells secrete cytokines upon activation.

Figure 3: Expanded human CD3+ T cells secrete cytokines upon activation. Expanded human T cells were stimulated with activation beads for 24 hours. Cell-free supernatant was harvested, and cytokine secretion was quantified via cytometric bead array. In response to stimulation, expanded T cells secrete IL-2, IL-10, TNF-α, and IFN-γ. Data shows the average (mean) of technical triplicates with SEM error bars.

T cell Dependent Cellular Cytotoxicity (TDCC)

Expanded human CD3+ T cells exhibit cytotoxicity toward tumor target cells.

Figure 4: Expanded human CD3+ T cells exhibit cytotoxicity toward tumor target cells. Expanded human CD3+ T cells were co-cultured with fluorescently-labeled Ramos cells and treated with either a human-reactive bispecific T cell engager antibody (CD3xCD19) or isotype control. Ramos target cell cytotoxicity was measured on a flow cytometer via viability dye incorporation. Data shows the average (mean) of technical triplicates, normalized with background subtraction, with SEM error bars.

Cryopreservation and storage

Expanded human CD3+ T cells are carefully cryopreserved using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 90%) upon thaw.

Cells should be stored at < -120 °C once they are received, such as within a liquid nitrogen tank (vapor phase).

Additional information

Available Size(s)

,

Cell Type

Format

Species

Tissue Type

Viability

> 90%

We are Committed to Ethical Practices

iQ Biosciences’ human primary cell products are lawfully obtained in accordance with Local, State, and Federal U.S. requirements, and the collection of cells complies with ethical requirements. Our cells are obtained from normal or disease patient volunteers participating in a donor program that is approved by an Institutional Review Board (IRB) or Human Subject Committee. A signed and witnessed consent form is obtained from donor volunteers prior to starting the collection protocol. Strict controls on personal identifiers of volunteers are in place in order to protect their privacy.

Tailored Experiments

Your research is important to us. Our scientists will work through each step of the process with you, including assay design, data analysis, and recommendations for future studies.

Streamlined Process

Simplify your workflow. Bypass the middle-man: at iQ Biosciences, you'll get immediate access to our biospecimen inventory, saving you both valuable time and money.

Expertise

We're experts- so you don't have to be. Augmenting years of experience in immunology and working with immune assays, our scientists stay current with the latest publications and technology.

Exceptional Service

We're here to help. We know the challenges you're facing: whether it be through expedited service or our complimentary consulting services, our team is dedicated to helping you reach your goals.

For US customers, we ship via FedEx Overnight Shipping. Shipping charges will vary per shipping address (based on ZIP code) and are estimated to be $140.

For international (non-US) customers, we work closely with you and our couriers to ensure all necessary documentation is in place for international shipments to significantly reduce the chance of delays at Customs. For the export of non-human primate samples, this includes preparing CITES permits, as well as any other documentation as required by country. Please submit an inquiry to orders@iqbiosciences.com for your estimated time of delivery and shipping charges.

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