- CD3+ T cells expanded from purified human T cells
- Expanded T cells are validated for retention of functional activity such as proliferation, cytokine production, and cytolytic activity
- Applications include a wide variety of safety assessment and functional assays
- Could be used for in vivo studies
- Carefully cryopreserved to ensure high viability (> 90%) upon thawing
- All orders come with an iQ Certificate of Analysis
Expanded Human CD3 T Cells
$350.00 – $1,210.00
Human CD3+ T cell Potential Application Summary
- Flow cytometry-based binding studies for target binding and cross-reactivity
- Large-scale potency assays to compare a panel of test articles for the ability to elicit T cell responses
- Adoptive transfer into immunodeficient recipient mice to support the in vivo evaluation of activity with therapeutic test articles
Expansion of Human CD3+ T cells
These human CD3+ T cells are meticulously expanded over a multi-day period using a combination of cytokines, expansion medium, and high quality T cell activation beads. The expansion process takes place in a controlled environment to ensure optimal conditions for cell growth and retained function.
Figure 1: T cell phenotype is maintained post-expansion. (a) CD3+ purity is maintained after expansion. (b) CD4+ T cell frequency decreases after expansion with a concordant increase in CD8+ T cell frequency. Data shows one representative sample.
Validation of Expanded Human CD3+ T cells
Our expanded human CD3+ T cells have undergone rigorous testing to validate their exceptional quality and functional ability as reflected by their capacity for robust proliferation, target cell killing, and cytokine production, in vitro. These cells may be used for various applications, including mechanistic research, therapeutic development, and innovative cellular assays.
Figure 2: CD8+ T cells within expanded human CD3+ T cells proliferate in response to stimulation. Expanded human T cells were stimulated with activation beads for 96 hours. Flow cytometry was used to assess the frequency of Ki-67+ (a marker of proliferation) cells within CD8+ T cells. Data shows the average (mean) of technical triplicates with SEM error bars.
Figure 3: Expanded human CD3+ T cells secrete cytokines upon activation. Expanded human T cells were stimulated with activation beads for 24 hours. Cell-free supernatant was harvested, and cytokine secretion was quantified via cytometric bead array. In response to stimulation, expanded T cells secrete IL-2, IL-10, TNF-α, and IFN-γ. Data shows the average (mean) of technical triplicates with SEM error bars.
T cell Dependent Cellular Cytotoxicity (TDCC)
Figure 4: Expanded human CD3+ T cells exhibit cytotoxicity toward tumor target cells. Expanded human CD3+ T cells were co-cultured with fluorescently-labeled Ramos cells and treated with either a human-reactive bispecific T cell engager antibody (CD3xCD19) or isotype control. Ramos target cell cytotoxicity was measured on a flow cytometer via viability dye incorporation. Data shows the average (mean) of technical triplicates, normalized with background subtraction, with SEM error bars.
Cryopreservation and storage
Expanded human CD3+ T cells are carefully cryopreserved using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 90%) upon thaw.
Cells should be stored at < -120 °C once they are received, such as within a liquid nitrogen tank (vapor phase).
We are Committed to Ethical Practices
iQ Biosciences’ human primary cell products are lawfully obtained in accordance with Local, State, and Federal U.S. requirements, and the collection of cells complies with ethical requirements. Our cells are obtained from normal or disease patient volunteers participating in a donor program that is approved by an Institutional Review Board (IRB) or Human Subject Committee. A signed and witnessed consent form is obtained from donor volunteers prior to starting the collection protocol. Strict controls on personal identifiers of volunteers are in place in order to protect their privacy.
For US customers, we ship via FedEx Overnight Shipping. Shipping charges will vary per shipping address (based on ZIP code) and are estimated to be $140.
For international (non-US) customers, we work closely with you and our couriers to ensure all necessary documentation is in place for international shipments to significantly reduce the chance of delays at Customs. For the export of non-human primate samples, this includes preparing CITES permits, as well as any other documentation as required by country. Please submit an inquiry to firstname.lastname@example.org for your estimated time of delivery and shipping charges.
BIOHUB INTERNATIONAL TRADE CO., LTD.
Address: Chuansha Rd #6619, Pudong, Shanghai, Zipcode: 201200 P.R.China
Cell & Gene BioSolutions Pvt. Ltd.
#478 C, SLV Complex, Raghavendra Swamy Mutt Road
Opp. Turahalli Water Tank, Turahalli, Subramanyapura Post
Uttarahalli Hobli, Bengaluru-560061, Karnataka, India
Phone: +91 97317 14670
Phone: +91 98809 25033