- NK cells purified from cynomolgus macaque PBMCs by magnetic selection
- Used for a wide variety of safety assessment and functional assays
- Carefully cryopreserved to ensure high viability (> 90%) upon thawing
- All orders come with an iQ Certificate of Analysis
Purified Cynomolgus Macaque Monkey NK Cells
- Additional information
- Donor Request
- iQ Experience
- Shipping Information
About the Cynomolgus Monkey (or Cynomolgus Macaque)
The cynomolgus macaque is the most commonly utilized non-human primate in biomedical research. According to some researchers, these monkeys are ideal models due to the 90-93% genetic similarity to and recent evolutionary divergence from humans. They are employed in numerous research areas, such as immunology, neuroscience, oncology, diabetes, and pharmacology due to their genetics and physiology.
Cynomolgus Macaque Monkey NK Cells
Cynomolgus NK cells make up approximately 2-18% of PBMCs in a typical adult monkey. These cells carry activating and inhibitory molecules that influence their activation status.
When activated, NK cells can lyse infected or transformed cells through natural cytotoxicity and antibody-dependent cellular cytotoxicity (ADCC).
NK Cell Application Summary
Purified cynomolgus NK cells are a good source of cells to study the biology of NK cells and their role in the immune system. They can be used for a variety of stimulation-dependent functional experiments to assess cytokine production and cytotoxicity.
With the current trend toward development of immuno-modulatory drugs, NK cells are an ideal source of cells to test cytotoxicity and cytokine production in the presence of therapeutic molecules. More importantly, NK cells can be used to assess toxicity and safety characteristics of these biologics in the presence of NK cell activation before first in-human trials. These cells are commonly employed in pre-clinical settings to ensure biologics are not eliciting unwanted functions, specifically from NK cells.
The positive isolation method of NK cells may lead to internalization of the marker that was used to isolate the cells. In most cases, these markers are only used for identification purposes and may not have any effect on function, but it will depend on the organism and function. Therefore, cells isolated using this method may be employed for functional or population characterization studies with the knowledge that the isolation marker may be internalized and not present.
NK Cell Purification
Collection of samples
Cynomolgus PBMCs were sourced from a responsible third party vendor that operate according to local regulations and laws.
Magnetic Positive Isolation of Cyno NK cells
To purify cynomolgus NK cells, PBMCs were incubated with antibodies against an NK cell-specific marker, which will vary depending on the species of the organism, and subsequently passed through a magnetic column. The cells that pass through are the non-NK cells, which can be used for other purposes, while the labeled NK cells are left bound to the column. These cells are subsequently eluted to obtain purified NK cells.
Cryopreservation and storage
Purified NK cells were cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 90%) after thawing.
Cells should be stored at < -120 °C once they are received, such as within a liquid nitrogen tank (vapor phase).
We are happy to accommodate the following donor requests if inventory is available. Feel free to inquire prior to ordering, or specify your interest in the "Order notes" section during checkout and we will follow-up with your shortly after your order is processed:
- Single or multiple donors for a particular product (SKU). Indicate number of vials from each individual donor
- Donor-matched PBMCs
- Donor(s) with a higher percentage of the cells of interest, i.e., B cells, CD14+ cells, CD4+ cells, CD8+ cells, or NK cells (for PBMC products only)
We are Committed to Ethical Practices
iQ Biosciences’ primary cell products from non-human primates are collected under approved IACUC protocol, which was developed in consultation with the Attending Veterinarian and is consistent with current veterinary standards. The collection facility is AAALACi and PHS accredited, and all animal housing, handling, and research protocols are consistent with standards set forth by the Animal Welfare Act, NIH's Policy on Humane Care and Use of Animals and the Guide for the Care and Use of Animals. All animal housing and handling is done in such a way to minimize stress and risk of injury to the animal.
For US customers, we ship via FedEx Overnight Shipping. Shipping charges will vary per shipping address (based on ZIP code) and are estimated to be $110.
For international (non-US) customers, we work closely with you and our couriers to ensure all necessary documentation is in place for international shipments to significantly reduce the chance of delays at Customs. For the export of non-human primate samples, this includes preparing CITES permits, as well as any other documentation as required by country. Please submit an inquiry to email@example.com for your estimated time of delivery and shipping charges.