- NK cells purified from cynomolgus macaque PBMCs by negative selection
- Used for a wide variety of safety assessment and functional assays
- Carefully cryopreserved to ensure high viability upon thawing
- All orders come with an iQ Certificate of Analysis
Purified Cynomolgus Macaque Monkey NK Cells
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About the Cynomolgus Macaque Monkey
The cynomolgus macaque is the most commonly used non-human primate used in biomedical research. According to some researchers, these monkeys are ideal models due to the 90-93% genetic similarity to and recent evolutionary divergence from humans. They are employed in numerous research areas, such as immunology, neuroscience, oncology, diabetes, and pharmacology due to their genetics and physiology.
Cynomolgus Macaque Monkey NK Cells
Cynomolgus NK cells make up approximately 2-18% of PBMCs in a typical adult monkey. These cells carry activating and inhibitory molecules that influence their activation status.
When activated, NK cells can lyse infected or transformed cells through natural cytotoxicity and antibody-dependent cellular cytotoxicity (ADCC).
NK Cell Application Summary
Purified cynomolgus NK cells are a good source of cells to study the biology of NK cells and their role in the immune system. They can be used for a variety of stimulation-dependent functional experiments to assess cytokine production and cytotoxicity.
With the current trend toward development of immuno-modulatory drugs, NK cells are an ideal source of cells to test cytotoxicity and cytokine production in the presence of therapeutic molecules. More importantly, NK cells can be used to assess toxicity and safety characteristics of these biologics in the presence of NK cell activation before first in-human trials. These cells are commonly employed in pre-clinical settings to ensure biologics are not eliciting unwanted functions, specifically from NK cells.
The negative isolation of NK cells leaves them untouched without any antibody binding to cell surface markers that may influence function. This method leaves all cell surface proteins eligible to be bound to antibodies or other molecules for functional or population characterization studies.
NK Cell Purification
Collection of samples
Cynomolgus PBMCs were sourced from a responsible third party vendor that operate according to local regulations and laws.
Negative Isolation of NK cells
To purify cynomolgus NK cells, PBMCs were incubated with antibodies against T cells, B cells, dendritic cells, monocytes, granulocytes, and erythrocytes, and subsequently run through a magnetic column. Cells labeled with the antibodies were bound to the column, while unlabeled cells, the NK cells, passed through.
Cryopreservation and storage
Purified NK cells were cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability after thawing.
Cells should be stored at <-120 °C once they are received, such as within a liquid nitrogen tank (vapor phase).
Please let us know in the notes section during checkout if you are interested in animal donor-matched PBMCs, as we carry these in stock.
If you would like to request multiple animal donors or the same animal donor, please specify this in the notes section as well.
Request a Cellular Products Fact Sheet for Non-Human PBMCs
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