Description

About the C57BL/6 Mouse

The C57BL/6 mouse is the most commonly-used inbred mouse strain available and is employed in numerous research areas, including immunology, genetics, diabetes and obesity, neurobiology, cardiovascular biology, and developmental biology. They are routinely used as the background strain for generation of congenics carrying modified genes and/or transgenes. These mice carry the H-2^b haplotype and their immune cells are commonly used in immune-based assays. Further, the C57BL/6 mouse is biased towards a Th1 cell-mediated immune response when challenged appropriately. Together, the C57BL/6 mouse has been extensively used to study numerous scientific questions and their cells (like these C57BL/6 mouse bone marrow cells) may offer many experimental benefits depending on the parameters of your experiments.

Application Summary for C57BL/6 Mouse Bone Marrow Cells

The bone marrow contains various types of cells with many beginning there as stem cells. Thus, mouse bone marrow cells are an excellent source for phenotyping and characterizing stem cells. Applications such as multi-parameter flow cytometry are routinely used to characterize and distinguish stem cells from other types or to discover new ones. In addition, these populations, if purified, can potentially be used to differentiate into specific cell types, depending on the culture conditions. Similarly, progenitor cells are also found in the bone marrow and are routinely used to differentiate into monocytes and macrophages. Together, bone marrow cells can be used to identify and study specific cell populations, as well as differentiate into cell types for functional assays.

Isolation of Bone Marrow Cells

Bone Marrow Collection and Isolation of Cells

Bone marrow is isolated responsibly from C57BL/6 mice at third party facilities. These facilities are vetted to ensure they operate according to local regulations and laws. Since the facility is local, the marrow is quickly transported to iQ’s facility for processing.

At our facility, bone marrow is carefully removed from the tissue and mechanically dissociated to obtain a single cell suspension under sterile conditions. The suspension is carefully passed through a filter to remove any aggregates, and subsequently depleted of red blood cells. The bone marrow is then frozen based on iQ Biosciences’ quality-controlled cryopreservation protocol to promote recoverability and viability once in the end user’s hands.

Cryopreservation and Storage

Our C57BL/6 mice bone marrow cells are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (typically > 70%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).

Immunophenotyping Data for C57BL/6 Mouse Bone Marrow Cells

To provide deeper insights into the cellular composition of our C57BL/6 mouse bone marrow cells, we have characterized the distribution of key immune cell populations. Below is a representative profile of cell types based on historical data:

*These percentages are for 6 to 10 week old mice. These values may differ depending on factors such as mouse age, gender, and environmental factors. Data is sourced from internal immunophenotyping analysis. Values are expressed as % of total leukocytes in each sample, unless otherwise specified.