Description

About the Sprague Dawley Rat

The Sprague Dawley rat is one of the two most widely-used outbred rat strains in biomedical research, including oncology, neurobiology, toxicology, pharmacology, aging, metabolism/nutrition, and neurobiology. Derived from the Wistar rat strain, the Sprague Dawley rat is also routinely employed in the field of immunology research as a model for autoimmune diseases and immunogenic challenges against pathogens. Thus, cells from Sprague Dawley rats may offer experimental advantages depending on the parameters of your experiments.

Application Summary for SD Rat Bone Marrow Cells

The bone marrow contains various types of cells with many beginning there as stem cells. Thus, SD rat bone marrow cells are an excellent source for phenotyping and characterizing stem cells. Applications such as multi-parameter flow cytometry are routinely used to characterize and distinguish stem cells from other types or to discover new ones. In addition, these populations, if purified, can potentially be used to differentiate into specific cell types, depending on the culture conditions. Similarly, progenitor cells are also found in the bone marrow and are routinely used to differentiate into monocytes and macrophages. Together, SD rat bone marrow cells can be used to identify and study specific cell populations, as well as differentiate into cell types for functional assays.

Isolation of Bone Marrow Cells

Bone Marrow Collection and Isolation of Cells

Bone marrow is isolated responsibly from SD rats at third party facilities. These facilities are vetted to ensure they operate according to local regulations and laws. Since the facility is local, the marrow is quickly transported to iQ’s facility for processing.

At our facility, bone marrow is carefully removed from the tissue and mechanically dissociated to obtain a single cell suspension under sterile conditions. The suspension is carefully passed through a filter to remove any aggregates, and subsequently depleted of red blood cells. The bone marrow is then frozen based on iQ Biosciences’ quality-controlled cryopreservation protocol to promote recoverability and viability once in the end user’s hands.

Cryopreservation and Storage

Our SD rat bone marrow cells are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (typically > 70%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).