Description

About the CD1 Mouse

The CD1 mouse is a commonly-used outbred strain for general multipurpose studies, but has also been extensively studied in oncology, toxicology, aging, and immunology.  Because they are outbred, the genetic profile of these mice is unknown.  However, their immune cells (like these CD1 mouse bone marrow cells) are routinely used in immune-based assays when genetic information is not specified.  This may offer benefits when comparing the immune response to that of cells from inbred mice.

Application Summary for CD1 Mouse Bone Marrow Cells

The bone marrow contains various types of cells with many beginning there as stem cells. Thus, CD1 Mouse bone marrow cells are an excellent source for phenotyping and characterizing stem cells. Applications such as multi-parameter flow cytometry are routinely used to characterize and distinguish stem cells from other types or to discover new ones. In addition, these populations, if purified, can potentially be used to differentiate into specific cell types, depending on the culture conditions. Similarly, progenitor cells are also found in the bone marrow and are routinely used to differentiate into monocytes and macrophages. Together, bone marrow cells can be used to identify and study specific cell populations, as well as differentiate into cell types for functional assays.

Isolation of Bone Marrow Cells

Bone Marrow Collection and Isolation of Cells

Bone marrow is isolated responsibly from CD1 mice at third party facilities. These facilities are vetted to ensure they operate according to local regulations and laws. Since the facility is local, the marrow is quickly transported to iQ’s facility for processing.

At our facility, bone marrow is carefully removed from the tissue and mechanically dissociated to obtain a single cell suspension under sterile conditions. The suspension is carefully passed through a filter to remove any aggregates, and subsequently depleted of red blood cells. The bone marrow is then frozen based on iQ Biosciences’ quality-controlled cryopreservation protocol to promote recoverability and viability once in the end user’s hands.

Cryopreservation and Storage

Our CD1 mouse bone marrow cells are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (typically > 70%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).