Description

About the C57BL/6 Mouse

The C57BL/6 mouse is the most commonly-used inbred mouse strain available and is employed in numerous research areas, including immunology, genetics, diabetes and obesity, neurobiology, cardiovascular biology, and developmental biology. They are routinely used as the background strain for generation of congenics carrying modified genes and/or transgenes. These mice carry the H-2^b haplotype and their immune cells are commonly used in immune-based assays. Further, the C57BL/6 mouse is biased towards a Th1 cell-mediated immune response when challenged appropriately. Together, the C57BL/6 mouse has been extensively used to study numerous scientific questions and their cells (like these C57BL/6 mouse splenocytes) may offer many experimental benefits depending on the parameters of your experiments.

Application Summary for C57BL/6 Mouse Splenocytes

C57BL/6 mouse splenocytes can be used for a wide variety of immunology-based applications. In-vitro immunoassays, such as ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to measure ADCC or CDC, and plaque assays are routinely performed using these cells. In addition, mouse splenocytes are also used in ex-vivo applications, such as cell population characterization and generation of genetically modified cells through retroviral infection.

Isolation of Splenocytes

Spleen Collection and Splenocyte Isolation

Spleens are isolated responsibly from C57BL/6 congenic mice at third party facilities. These facilities are vetted to ensure they operate according to local regulations and laws. Since the facility is local, the tissue is quickly transported to iQ’s facility for processing.

Once at our facility, the splenic tissue is carefully dissociated through a gentle mechanical process to generate a single cell suspension under sterile conditions. The suspension is then carefully passed through a filter to remove any fat and/or connective tissue and subsequently depleted of red blood cells in a manner that is not damaging to the other cells. The purified splenocytes are then frozen based on iQ Biosciences’ quality-controlled cryopreservation protocol to promote recoverability and viability once in the end user’s hands.

Expression of CD45.1

Splenocytes are isolated from tissue of mice that have been verified for the CD45.1 allele by genotyping.

Cryopreservation and Storage

Our C57BL/6 mouse splenocytes are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (typically > 70%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).

Immunophenotyping Data for C57BL/6 Mouse Splenocytes

To provide deeper insights into the cellular composition of our C57BL/6 mouse splenocytes, we have characterized the distribution of key immune cell populations. Below is a representative profile of cell types based on historical data:

*These percentages are for 6 to 10 week old mice. These values may differ depending on factors such as mouse age, gender, and environmental factors. Data is sourced from internal immunophenotyping analysis. Values are expressed as % of total leukocytes in each sample, unless otherwise specified.