Name: BALB/c Mouse Splenocytes
SKU: IQB-MSP1xx
Availability: InStock

BALB/c Mouse Splenocytes

$300.00 – $430.00

High-quality splenocytes from the BALB/c mouse, a commonly-used inbred mouse strain
High viability (typically > 70%) after thawing
Used for a wide variety of immunology-based applications, including in-vitro immunoassays and ex-vivo applications
All orders come with an iQ Certificate of Analysis
Normally ships out same business day

SKU: IQB-MSP1xx Categories: BALB/c Mouse, Mouse, Spleen Tags: BALB/c, mouse. splenocytes

Description

About the BALB/c Mouse

The BALB/c mouse is a commonly-used inbred mouse strain for the study of autoimmune diseases and pathogen-driven immune response. They carry the H-2^dhaplotype and their immune cells are routinely used in immune-based assays. Based on early studies, the BALB/c mouse is biased towards a Th2 antibody-mediated immune response when challenged appropriately. Thus, the BALB/c mouse is well-studied in the field of immunology and their cells (like BALB/c mouse splenocytes) may offer experimental advantages depending on the parameters of your experiments.

Application Summary for BALB/c Mouse Splenocytes

BALB/c mouse splenocytes can be used for a wide variety of immunology-based applications. In-vitro immunoassays, such as ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to measure ADCC or CDC, and plaque assays are routinely performed using these cells. In addition, mouse splenocytes are also used in ex vivo applications, such as cell population characterization and generation of genetically modified cells through retroviral infection.

Isolation of Splenocytes

Spleen Collection and Splenocyte Isolation

Spleens are isolated responsibly from BALB/c mice at third party facilities. These facilities are vetted to ensure they operate according to local regulations and laws. Since the facility is local, the tissue is quickly transported to iQ’s facility for processing.

Once at our facility, the splenic tissue is carefully dissociated through a gentle mechanical process to generate a single cell suspension under sterile conditions. The suspension is then carefully passed through a filter to remove any fat and/or connective tissue and subsequently depleted of red blood cells in a manner that is not damaging to the other cells. The purified splenocytes are then frozen based on iQ Biosciences’ quality-controlled cryopreservation protocol to promote recoverability and viability once in the end user’s hands.

Cryopreservation and Storage

Our BALB/c mouse splenocytes are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (typically > 70%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).

Additional information

Available Size(s)	25 million cells / vial, 50 million cells / vial
Cell Type	Splenocytes
Format	Frozen
Species	Mouse
Tissue Type	Spleen
Viability	Typically > 70%

For US customers, we ship via FedEx Overnight Shipping. Shipping charges will vary per shipping address (based on ZIP code) and are estimated to be $120.

For international (non-US) customers, we work closely with you and our couriers to ensure all necessary documentation is in place for international shipments to significantly reduce the chance of delays at Customs. For the export of non-human primate samples, this includes preparing CITES permits, as well as any other documentation as required by country. Please submit an inquiry to orders@iqbiosciences.com for your estimated time of delivery and shipping charges.

Austria

Hölzel Diagnostika Handels GmbH
Tel: +49 221 126 02 66
Email: info@hoelzel.de
Web: https://www.hoelzel-biotech.com/

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Cedarlane
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BIOHUB INTERNATIONAL TRADE CO., LTD.
上海起发实验试剂有限公司
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European Union

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tebu-bio
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Or Find a local contact

Germany

Hölzel Diagnostika Handels GmbH
Tel: +49 221 126 02 66
Email: info@hoelzel.de
Web: https://www.hoelzel-biotech.com/

Zageno
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Ireland

2B Scientific Ltd
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Fax: +44(0) 1869 238034
Email: sales@2BScientific.com
Web: https://www.2bscientific.com

India

Cell & Gene BioSolutions Pvt. Ltd.
#478 C, SLV Complex, Raghavendra Swamy Mutt Road
Opp. Turahalli Water Tank, Turahalli, Subramanyapura Post
Uttarahalli Hobli, Bengaluru-560061, Karnataka, India
Phone: +91 97317 14670
Phone: +91 98809 25033
Email: info@cgbios.com
Web: www.cgbios.com

Japan