Description

About the BALB/c Mouse

The BALB/c mouse is a commonly-used inbred mouse strain for the study of autoimmune diseases and pathogen-driven immune response. They carry the H-2^d haplotype and their immune cells are routinely used in immune-based assays. Based on early studies, the BALB/c mouse is biased towards a Th2 antibody-mediated immune response when challenged appropriately. Thus, the BALB/c mouse is well-studied in the field of immunology and their cells (like BALB/c mouse splenocytes) may offer experimental advantages depending on the parameters of your experiments.

Application Summary for BALB/c Mouse Splenocytes

BALB/c mouse splenocytes can be used for a wide variety of immunology-based applications. In-vitro immunoassays, such as ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to measure ADCC or CDC, and plaque assays are routinely performed using these cells. In addition, mouse splenocytes are also used in ex vivo applications, such as cell population characterization and generation of genetically modified cells through retroviral infection.

Isolation of Splenocytes

Spleen Collection and Splenocyte Isolation

Spleens are isolated responsibly from BALB/c mice at third party facilities. These facilities are vetted to ensure they operate according to local regulations and laws. Since the facility is local, the tissue is quickly transported to iQ’s facility for processing.

Once at our facility, the splenic tissue is carefully dissociated through a gentle mechanical process to generate a single cell suspension under sterile conditions. The suspension is then carefully passed through a filter to remove any fat and/or connective tissue and subsequently depleted of red blood cells in a manner that is not damaging to the other cells. The purified splenocytes are then frozen based on iQ Biosciences’ quality-controlled cryopreservation protocol to promote recoverability and viability once in the end user’s hands.

Cryopreservation and Storage

Our BALB/c mouse splenocytes are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (typically > 70%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).

Immunophenotyping Data for BALB/c Mouse Splenocytes

To provide deeper insights into the cellular composition of our BALB/c mouse splenocytes, we have characterized the distribution of key immune cell populations. Below is a representative profile of cell types based on historical data:

*These percentages are for 6 to 10 week old mice. These values may differ depending on factors such as mouse age, gender, and environmental factors. Data is sourced from internal immunophenotyping analysis. Values are expressed as % of total leukocytes in each sample, unless otherwise specified.