Purified Beagle Canine CD14+ Cells

Description

About the Beagle Canine

The Beagle canine is the most commonly-used breed of dog for the study of cardiology, cancer, microbial pathogenesis, and immunology. Specifically, they have been used to study the pathogenesis of and response to Leishmania infantum, an intracellular parasite that can pose public health concerns. In these experiments, PBMCs from beagles that are cultured in the presence of L. infantum can respond to the virus by proliferating. Therefore, beagle immune cells may be used to study other types of immune responses and may also offer experimental advantages depending on the parameters of your experiments.

Beagles are also used in toxicity studies for a variety of therapeutics developed by pharmaceutical and biotech companies.

Application Summary for Beagle Canine CD14+ Cells

Purified beagle CD14+ cells are a good source of monocytes to study their biology since they make up approximately 50% of CD14+ cells. These isolated cells can be used to examine the differentiation and development of the beagle monocyte population to the macrophage or dendritic cell subsets depending on the cytokine environment. Further, these differentiated cells and monocytes can also be used for stimulation-dependent functional experiments to assess cytokine production and antigen presentation.

Similarly, isolated CD14+ cells are a good source of mature neutrophils to study their biology, in particular their functional capabilities. They are capable of secreting enzymes, as well as performing phagocytosis in response to foreign organisms.

With the current trend toward development of immuno-modulatory drugs, CD14+ cells are an ideal source of cells to test inflammatory factor production in the presence of therapeutic molecules. More importantly, these beagle CD14+ cells can be used to assess toxicity and safety characteristics of these biologics before first in-human trials as these cells can be employed in pre-clinical settings to ensure the biologics are not eliciting unwanted monocyte or neutrophil-mediated functions.

Beagle Canine CD14+ Cell Purification

Collection of Samples

Beagle PBMCs were isolated from blood sourced from a responsible third party vendor that operate according to local regulations and laws.

Magnetic Positive Isolation of CD14+ Cells

To purify beagle CD14+ cells, PBMCs were incubated with antibodies against CD14 and subsequently passed through a magnetic column. The cells that pass through are the non-CD14+ cells, which can be used for other purposes, while the labeled CD14+ cells are left bound to the column. These cells are subsequently eluted to obtain purified CD14+ cells.

Cryopreservation and Storage

Our purified CD14+ cells were cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 80%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).