Description

About the Wistar Rat

The Wistar rat is one of the two most routinely-used albino rat strains in biomedical research. They are commonly used in many fields of research, including oncology, aging, pharmacology, toxicology, and metabolism/nutrition. They are also used in immunology as models for infectious diseases and autoimmune responses. Depending on the parameters of your experiments, cells from Wistar rats, like Wistar rat splenocytes, may offer experimental advantages.

Application Summary for Wistar Rat Splenocytes

Wistar rat splenocytes can be used for a wide variety of immunology-based applications. In-vitro immunoassays, such as ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to measure ADCC or CDC, and plaque assays are routinely performed using these cells. In addition, rat splenocytes are also used in ex-vivo applications, such as cell population characterization and generation of genetically modified cells through retroviral infection.

Isolation of CD45.1 Splenocytes

Spleen Collection and Splenocyte Isolation

Spleens are isolated responsibly from Wistar rats at third party facilities. These facilities are vetted to ensure they operate according to local regulations and laws. Since the facility is local, the tissue is quickly transported to iQ’s facility for processing.

Once at our facility, the splenic tissue is carefully dissociated through a gentle mechanical process to generate a single cell suspension under sterile conditions. The suspension is then carefully passed through a filter to remove any fat and/or connective tissue and subsequently depleted of red blood cells in a manner that is not damaging to the other cells. The purified splenocytes are then frozen based on iQ Biosciences’ quality-controlled cryopreservation protocol to promote recoverability and viability once in the end user’s hands.

Cryopreservation and Storage

Our Wistar rat splenocytes are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (typically > 70%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).