Rhesus Monkey Peripheral Blood Mononuclear Cells (PBMCs)

$355.00$550.00

Features

  • High-quality rhesus macaque PBMCs sourced responsibly from a vetted third party facility
  • High viability (> 90%) after thawing
  • Used for a wide variety of safety assessment and functional assays
  • All orders come with an iQ Certificate of Analysis
  • Normally ships out same business day

Contact us for info on bulk discounts

Description

About the Rhesus Monkey (Rhesus Macaque)

Rhesus macaques are one of the most commonly utilized non-human primates in biomedical research. They are employed in numerous research areas, such as immunology, neuroscience, oncology, infectious disease, and toxicology due to their physiology.

Application Summary for Rhesus Monkey PBMCs

Rhesus monkey PBMCs can be used for a wide variety of safety assessment and functional studies. They are commonly employed in pre-clinical settings to ensure biologics are not eliciting unwanted functions, such as cytokine release and toxicity. They are also used in many immunology-based applications, such as ELISPOT, cytokine production assays, and cytotoxicity assays to measure ADCC or CDC in response to biologics. In addition, rhesus monkey PBMCs can also be used in ex-vivo applications for cell population characterization.

Isolation of PBMCs

Peripheral blood is obtained from responsible facilities that have many years of experience collecting samples. Since the facilities are local, the blood is quickly transported to iQ Bioscience’s laboratory for processing.

Once at our lab, PBMCs are isolated by performing density centrifugation with Ficoll, a high molecular weight sucrose solution, to remove red blood cells under sterile conditions. Depending on the species (human or non-human) of blood, either 1.077 or 1.084 g/ml Ficoll is used, resulting in a perfect layer of mononuclear cells that are separated from plasma, platelets, granulocytes, and erythrocytes. Additional quality control steps are taken to isolate and prepare PBMCs that ensure the highest viability for cryopreservation and downstream experimental applications.

Cryopreservation and Storage

Our rhesus PBMCs are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 90%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).

Quality Control Process

All of our non-human primate donors have been tested for Herpes B virus, Simian Retrovirus (SRV), and tuberculosis. Additionally, we implement random sampling per lot to test for viability and cell counts to ensure they meet specifications, which are recorded on the Certificate of Analysis that is included with each shipment. Cell counts are obtained using a manual hemocytometer and then cross-referenced with an automated cell counter. Each lot is also characterized for unique cell populations by immunophenotyping, in which the results are also recorded on the Certificate of Analysis.

Immunophenotyping of rhesus PBMCs

Figure 1. Flow profiles of T cells (CD3, CD4 gated on CD3+ population, and CD8 gated on CD3+ population), monocyte (CD14), B cell (CD20), and NK cell (CD56) populations in rhesus PBMCs. The specific immune cell subset and its percentage is listed on the top right of the flow profile. Briefly, rhesus PBMCs were stained with antibodies for CD3, CD4, CD8, CD14, CD20, and CD56, followed by flow cytometric analysis.

Additional information

Available Size(s)

Format

Cell Type

Tissue Type

Species

Viability

> 90%

Donor Request

We are happy to accommodate the following donor requests if inventory is available. Feel free to inquire prior to ordering, or specify your interest in the "Order notes" section during checkout, and we will follow-up with you shortly after your order is processed, if necessary:

  • Single or multiple donors for a particular product (SKU). Indicate the number of vials from each individual donor
  • Donor(s) with a higher percentage of the cells of interest (e.g., B cells, CD4+ cells, CD8+ cells, NK cells, etc.)

Shipping Information

For US customers, we ship via FedEx Overnight Shipping. Shipping charges will vary per shipping address (based on ZIP code) and are estimated to be $140.

For international (non-US) customers, we work closely with you and our couriers to ensure all necessary documentation is in place for international shipments to significantly reduce the chance of delays at Customs. For the export of non-human primate samples, this includes preparing CITES permits, as well as any other documentation as required by country. Please submit an inquiry to orders@iqbiosciences.com for your estimated time of delivery and shipping charges.

Distributors

Austria Austria FlagHölzel Diagnostika Handels GmbH

+49 221 126
Canada Canada flagCedarlane

+1 (289) 288-0001
+1 (800) 268-5058
+1 (289) 288-0020
China China FlagBIOHUB INTERNATIONAL TRADE CO., LTD. 上海起发实验试剂有限公司
Address: Chuansha Rd #6619, Pudong, Shanghai, Zipcode: 201200 P.R.China

0086-021-50724187
+86-15921799099
0086-021-50724961
GermanyGermany FlagHölzel Diagnostika Handels GmbH

+49 221 126 02 66

Zageno

Ireland Ireland Flag 2B Scientific Ltd

+44(0) 1869 238033
+44(0) 1869 238034
India India FlagCell & Gene BioSolutions Pvt. Ltd. #478 C, SLV Complex, Raghavendra Swamy Mutt Road Opp. Turahalli Water
Tank, Turahalli, Subramanyapura Post Uttarahalli Hobli, Bengaluru-560061, Karnataka, India

+91 97317 14670
+91 98809 25033
JapanJapan FlagContacts:

+81 (03) 5632 9610
+81 (03) 5632 9619
Qatar Singapore FlagSedeer Medical Services and Trading LLC

+974 4434 9191
Singapore Singapore FlagOmnicell Pte Ltd

+65 6747 0201
South Korea South Korea FlagBioClone

+82-2-2690-0058
Switzerland Austria Flag Hölzel Diagnostika Handels GmbH

+49 221 126 02 66
United States United States Flag Fisher Scientific

1-800-766-7000

Quartzy

VWR International

1-800-932-5000

Zageno

United Kingdom United Kingdom Flag 2B Scientific Ltd

+44(0) 1869 38033
+44(0) 1869 238034

 https://www.2bscientific.com
Caltag Medsystems Ltd.

+44 (0)1280 827460
+44 (0)1280 827466

Tebubio

+44 (0)1733 421880
+44 (0)1733 421882

 https://www.tebubio.com
Zageno

Taiwan Taiwan Flag Hycell International Co. Ltd.

Ordering Process

Step 1

Frame 1000003682
Search for products online

Access our easy-to-navigate catalog and find the cell types from the species you’re looking for. Add 
your desired quantities to the cart.

Step 2

checkout
Checkout

Once you’re done shopping, complete the easy checkout process. We accept credit card payments for immediate billing, as well as purchase orders via file upload.

Step 3

receive
Receive your product – FAST!

For your convenience, most of our products ship the same day with overnight delivery, as long as your order is submitted by 2PM Pacific Time Monday – Thursday. Be prepared to receive your cryopreserved product.

Citations

Go to Top