- High-quality beagle canine PBMCs, a commonly-used dog breed in research
- High viability (> 80%) after thawing
- Used for a wide variety of immunology-based applications, including in-vitro immunoassays and ex-vivo applications
- All orders come with an iQ Certificate of Analysis
- Normally ships out same business day
Beagle Canine Peripheral Blood Mononuclear Cells (PBMCs)
$475.00 – $800.00
About the Beagle Canine
The Beagle canine is the most commonly-used breed of dog for the study of cardiology, cancer, microbial pathogenesis, and immunology. Specifically, they have been used to study the pathogenesis of and response to Leishmania infantum, an intracellular parasite that can pose public health concerns. In these experiments, PBMCs from beagles that are cultured in the presence of L. infantum can respond to the virus by proliferating. Therefore, beagle PBMCs may be used to study other types of immune responses and may also offer experimental advantages depending on the parameters of your experiments.
Beagles are also used in toxicity studies for a variety of therapeutics developed by pharmaceutical and biotech companies.
Application Summary for Beagle Canine PBMCs
Beagle canine PBMCs can be used for a wide variety of immunology-based applications. In-vitro immunoassays, such as proliferation and cytotoxicity assays are routinely performed using these cells. In addition, beagle canine PBMCs are used in ex-vivo applications, such as cell population characterization based on immunophenotyping. Finally, beagle canine PBMCs are a good source of cells to test cross-reactivity of test molecules across a range of animals.
Isolation of PBMCs
Peripheral blood is obtained from responsible facilities that have many years of experience collecting samples. Since the facilities are local, the blood is quickly transported to iQ Bioscience’s laboratory for processing.
Once at our lab, PBMCs are isolated by performing density centrifugation with Ficoll, a high molecular weight sucrose solution, to remove red blood cells under sterile conditions. Depending on the species (human or non-human) of blood, either 1.077 or 1.084 g/ml Ficoll is used, resulting in a perfect layer of mononuclear cells that are separated from plasma, platelets, granulocytes, and erythrocytes. Additional quality control steps are taken to isolate and prepare PBMCs that ensure the highest viability for cryopreservation and downstream experimental applications.
Cryopreservation and Storage
Our canine PBMCs are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 80%) after thawing.
Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).
For US customers, we ship via FedEx Overnight Shipping. Shipping charges will vary per shipping address (based on ZIP code) and are estimated to be $110.
For international (non-US) customers, we work closely with you and our couriers to ensure all necessary documentation is in place for international shipments to significantly reduce the chance of delays at Customs. For the export of non-human primate samples, this includes preparing CITES permits, as well as any other documentation as required by country. Please submit an inquiry to firstname.lastname@example.org for your estimated time of delivery and shipping charges.