Description

About the Beagle Canine

The Beagle canine is the most commonly-used breed of dog for the study of cardiology, cancer, microbial pathogenesis, and immunology. Specifically, they have been used to study the pathogenesis of and response to Leishmania infantum, an intracellular parasite that can pose public health concerns. In these experiments, PBMCs from beagles that are cultured in the presence of L. infantum can respond to the virus by proliferating. Therefore, beagle PBMCs may be used to study other types of immune responses and may also offer experimental advantages depending on the parameters of your experiments.

Beagles are also used in toxicity studies for a variety of therapeutics developed by pharmaceutical and biotech companies.

Application Summary for Beagle Canine PBMCs

Beagle canine PBMCs can be used for a wide variety of immunology-based applications. In-vitro immunoassays, such as proliferation and cytotoxicity assays are routinely performed using these cells. In addition, beagle canine PBMCs are used in ex-vivo applications, such as cell population characterization based on immunophenotyping.  Finally, beagle canine PBMCs are a good source of cells to test cross-reactivity of test molecules across a range of animals.

Isolation of PBMCs

Peripheral blood is obtained from responsible facilities that have many years of experience collecting samples. Since the facilities are local, the blood is quickly transported to iQ Bioscience’s laboratory for processing.

Once at our lab, PBMCs are isolated by performing density centrifugation with Ficoll, a high molecular weight sucrose solution, to remove red blood cells under sterile conditions. Depending on the species (human or non-human) of blood, either 1.077 or 1.084 g/ml Ficoll is used, resulting in a perfect layer of mononuclear cells that are separated from plasma, platelets, granulocytes, and erythrocytes. Additional quality control steps are taken to isolate and prepare PBMCs that ensure the highest viability for cryopreservation and downstream experimental applications.

Cryopreservation and Storage

Our canine PBMCs are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 80%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).