- High-quality PBMCs from the C57BL/6 mouse, the most commonly-used inbred mouse strain
- High viability (> 80%) after thawing
- Used for a wide variety of immunology-based applications, including in-vitro immunoassays and ex-vivo applications
- All orders come with an iQ Certificate of Analysis
- Normally ships out same business day
C57BL/6 Mouse Peripheral Blood Mononuclear Cells (PBMCs)
About the C57BL/6 Mouse
The C57BL/6 mouse is the most commonly-used inbred mouse strain and is employed in numerous research areas, including immunology, genetics, diabetes and obesity, neurobiology, cardiovascular biology, and developmental biology. They are routinely used as the background strain for generation of congenics carrying modified genes and/or transgenes. These mice carry the H-2b haplotype and their immune cells are commonly used in immune-based assays. Further, the C57BL/6 mouse is biased towards a Th1 cell-mediated immune response when challenged appropriately. Together, the C57BL/6 mouse has been extensively used to study numerous scientific questions and their cells (like these C57BL/6 mouse PBMCs) may offer many experimental benefits depending on the parameters of your experiments.
Application Summary for C57BL/6 Mouse PBMCs
C57BL/6 Mouse PBMCs can be used for a wide variety of immunology-based applications. In-vitro immunoassays, such as ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to measure ADCC or CDC, and plaque assays are routinely performed using these cells. In addition, PBMCs are also used in ex-vivo applications, such as cell population characterization and generation of genetically modified cells through retroviral infection.
Isolation of PBMCs
Peripheral blood is obtained from responsible facilities that have many years of experience collecting samples. Since the facilities are local, the blood is quickly transported to iQ Bioscience’s laboratory for processing.
Once at our lab, PBMCs are isolated by performing density centrifugation with Ficoll, a high molecular weight sucrose solution, to remove red blood cells under sterile conditions. Depending on the species (human or non-human) of blood, either 1.077 or 1.084 g/ml Ficoll is used, resulting in a perfect layer of mononuclear cells that are separated from plasma, platelets, granulocytes, and erythrocytes. Additional quality control steps are taken to isolate and prepare PBMCs that ensure the highest viability for cryopreservation and downstream experimental applications.
Cryopreservation and Storage
Our mouse PBMCs are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 80%) after thawing.
Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).
For US customers, we ship via FedEx Overnight Shipping. Shipping charges will vary per shipping address (based on ZIP code) and are estimated to be $110.
For international (non-US) customers, we work closely with you and our couriers to ensure all necessary documentation is in place for international shipments to significantly reduce the chance of delays at Customs. For the export of non-human primate samples, this includes preparing CITES permits, as well as any other documentation as required by country. Please submit an inquiry to firstname.lastname@example.org for your estimated time of delivery and shipping charges.