- High-quality splenocytes from the Sprague Dawley rat, a commonly-used outbred rat strain
- High viability (typically > 70%) after thawing
- Used for a wide variety of immunology-based applications, including in-vitro immunoassays and ex-vivo applications
- All orders come with an iQ Certificate of Analysis
- Normally ships out same business day
SD Rat Splenocytes
$190.00 – $285.00
About the Sprague Dawley Rat
The Sprague Dawley rat is one of the two most widely-used outbred rat strains in biomedical research, including oncology, neurobiology, toxicology, pharmacology, aging, metabolism/nutrition, and neurobiology. Derived from the Wistar rat strain, the Sprague Dawley rat is also routinely employed in the field of immunology research as a model for autoimmune diseases and immunogenic challenges against pathogens. Thus, cells from Sprague Dawley rats, like SD rat splenocytes, may offer experimental advantages depending on the parameters of your experiments.
Application Summary for SD Rat Splenocytes
SD rat splenocytes can be used for a wide variety of immunology-based applications. In-vitro immunoassays, such as ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to measure ADCC or CDC, and plaque assays are routinely performed using these cells. In addition, rat splenocytes are also used in ex-vivo applications, such as cell population characterization and generation of genetically modified cells through retroviral infection.
Isolation of CD45.1 Splenocytes
Spleen Collection and Splenocyte Isolation
Spleens are isolated responsibly from SD rats at third party facilities. These facilities are vetted to ensure they operate according to local regulations and laws. Since the facility is local, the tissue is quickly transported to iQ’s facility for processing.
Once at our facility, the splenic tissue is carefully dissociated through a gentle mechanical process to generate a single cell suspension under sterile conditions. The suspension is then carefully passed through a filter to remove any fat and/or connective tissue and subsequently depleted of red blood cells in a manner that is not damaging to the other cells. The purified splenocytes are then frozen based on iQ Biosciences’ quality-controlled cryopreservation protocol to promote recoverability and viability once in the end user’s hands.
Cryopreservation and Storage
Our SD rat splenocytes are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (typically > 70%) after thawing.
Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).
Typically > 70%
For US customers, we ship via FedEx Overnight Shipping. Shipping charges will vary per shipping address (based on ZIP code) and are estimated to be $110.
For international (non-US) customers, we work closely with you and our couriers to ensure all necessary documentation is in place for international shipments to significantly reduce the chance of delays at Customs. For the export of non-human primate samples, this includes preparing CITES permits, as well as any other documentation as required by country. Please submit an inquiry to firstname.lastname@example.org for your estimated time of delivery and shipping charges.