- CD4 T cells purified from rhesus macaque PBMCs by negative selection
- Used for a wide variety of safety assessment and functional assays
- Carefully cryopreserved to ensure high viability upon thawing
- All orders come with an iQ Certificate of Analysis
Purified Rhesus Macaque Monkey CD4 T Cells
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About the Rhesus Macaque Monkey
The rhesus macaque (and rhesus monkey PBMCs) is one of the most commonly used non-human primate in biomedical research. They are employed in numerous research areas, such as immunology, neuroscience, oncology, infectious disease, and toxicology due to their physiology.
Rhesus Macaque Monkey CD4 T Cells
Rhesus CD4 T cells make up approximately 25-47% of PBMCs in a typical adult monkey. These cells carry the CD4 protein marker and the T cell receptor (TCR), which recognizes foreign antigens presented by major histocompatibility complex class I molecules on antigen presenting cells to activate them.
CD4 T cells develop in the thymus to become mature T cells and exit the thymus to reside and encounter foreign pathogens in lymphoid tissues.
When activated, CD4 T cells become T helper subsets to secrete cytokines to boost the immune response. Depending on the helper subset and cytokine released, the humoral (antibody-based) or cell-mediated immune response is elaborated.
CD4 T Cell Application Summary
Purified rhesus CD4 T cells are a good source of cells to study the biology of CD4 T cells and their role in the immune system. They can be used for a variety of stimulation-dependent functional experiments to assess cytotoxicity and proliferation.
With the current trend toward development of immuno-modulatory drugs, CD4 T cells are an ideal source of cells to test cytokine response in the presence of therapeutic molecules. More importantly, rhesus T cells can be used to assess toxicity and safety characteristics of these biologics in the presence of CD4 T cell activation before first in-human trials. These cells are commonly employed in pre-clinical settings to ensure biologics are not eliciting unwanted functions, specifically from CD4 T cells.
The negative isolation of CD4 T cells leaves them untouched without any antibody binding to cell surface markers that may influence function. This method leaves all cell surface proteins eligible to be bound to antibodies or other molecules for functional or population characterization studies.
CD4 T Cell Purification
Collection of samples
Rhesus PBMCs were sourced from a responsible third party vendor that operate according to local regulations and laws.
Negative Isolation of CD4 T cells
To purify rhesus CD4 T cells, PBMCs were incubated with antibodies against CD8 T cells, B cells, NK cells, dendritic cells, monocytes, granulocytes, and erythrocytes, and subsequently run through a magnetic column. Cells labeled with the antibodies were bound to the column, while unlabeled cells, the CD4 T cells, passed through.
Cryopreservation and storage
Purified CD4 T cells were cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability after thawing.
Cells should be stored at <-120 °C once they are received, such as within a liquid nitrogen tank (vapor phase).
10 million cells / vial, 5 million cells / vial
Normal Peripheral Blood
Please let us know in the notes section during checkout if you are interested in animal donor-matched PBMCs, as we carry these in stock.
If you would like to request multiple animal donors or the same animal donor, please specify this in the notes section as well.
Request a Cellular Products Fact Sheet for Non-Human PBMCs
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