DBA/2 Mouse Peripheral Blood Mononuclear Cells (PBMCs)

DBA/2 Mouse Peripheral Blood Mononuclear Cells (PBMCs)

$700.00

  • High-quality PBMCs from the DBA/2 mouse, a widely-used inbred mouse strain
  • High viability (> 80%) after thawing
  • Used for a wide variety of immunology-based applications, including in-vitro immunoassays and ex-vivo applications
  • All orders come with an iQ Certificate of Analysis
  • Normally ships out same business day
SKU: IQB-MPB5xx Categories: , , Tags: , ,

Description

About the DBA/2 Mouse

The DBA/2 mouse is a widely-used inbred mouse strain to study cardiovascular biology, neurobiology, and immunology. Often contrasted to the C57BL/6 mouse, the DBA/2 mouse carries the H-2d haplotype and their immune cells are routinely used in immune-based assays. Thus, the DBA/2 mouse is well employed in the field of immunology and their cells (like these DBA/2 Mouse PBMCs) may offer experimental advantages depending on the parameters of your experiments.

Application Summary for DBA/2 Mouse PBMCs

DBA/2 Mouse PBMCs can be used for a wide variety of immunology-based applications. In-vitro immunoassays, such as ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to measure ADCC or CDC, and plaque assays are routinely performed using these cells. In addition, PBMCs are also used in ex-vivo applications, such as cell population characterization and generation of genetically modified cells through retroviral infection.

Isolation of PBMCs

Peripheral blood is obtained from responsible facilities that have many years of experience collecting samples. Since the facilities are local, the blood is quickly transported to iQ Bioscience’s laboratory for processing.

Once at our lab, PBMCs are isolated by performing density centrifugation with Ficoll, a high molecular weight sucrose solution, to remove red blood cells under sterile conditions. Depending on the species (human or non-human) of blood, either 1.077 or 1.084 g/ml Ficoll is used, resulting in a perfect layer of mononuclear cells that are separated from plasma, platelets, granulocytes, and erythrocytes. Additional quality control steps are taken to isolate and prepare PBMCs that ensure the highest viability for cryopreservation and downstream experimental applications.

Cryopreservation and Storage

Our mouse PBMCs are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 80%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).

Additional information

Available Size(s)

Cell Type

Format

Species

Tissue Type

Viability

> 80%

Request a Cellular Products Fact Sheet for Non-Human PBMCs

Interested in learning more about this Cellular Product? Complete the form below to request a Fact Sheet.





Tailored Experiments

Your research is important to us. Our scientists will work through each step of the process with you, including assay design, data analysis, and recommendations for future studies.

Streamlined Process

Simplify your workflow. Bypass the middle-man: at iQ Biosciences, you'll get immediate access to our biospecimen inventory, saving you both valuable time and money.

Expertise

We're experts- so you don't have to be. Augmenting years of experience in immunology and working with immune assays, our scientists stay current with the latest publications and technology.

Exceptional Service

We're here to help. We know the challenges you're facing: whether it be through expedited service or our complimentary consulting services, our team is dedicated to helping you reach your goals.

 

For US customers, we ship via FedEx Overnight Shipping. Shipping charges will vary per shipping address (based on ZIP code) and are estimated to be $110.

For international (non-US) customers, we work closely with you and our couriers to ensure all necessary documentation is in place for international shipments to significantly reduce the chance of delays at Customs. For the export of non-human primate samples, this includes preparing CITES permits, as well as any other documentation as required by country. Please submit an inquiry to orders@iqbiosciences.com for your estimated time of delivery and shipping charges.

Fisher Scientific (United States)
Tel: 1-800-766-7000
Web: https://www.fishersci.com

VWR International (United States)
Tel: 1-800-932-5000
Web: https://www.vwr.com

Cosmo Bio Co., Ltd (Japan)
Tel: 03-5632-9610
Fax: 03-5632-9619
Email: nsmail@cosmobio.co.jp
Web: https://www.cosmobio.co.jp

BioClone (South Korea)
Tel: +82-2-2690-0058
Email: bioclone@bioclone.co.kr
Web: http://www.bioclone.co.kr