Description
About the DBA/2 Mouse
The DBA/2 mouse is a widely-used inbred mouse strain to study cardiovascular biology, neurobiology, and immunology. Often contrasted to the C57BL/6 mouse, the DBA/2 mouse carries the H-2d haplotype and their immune cells are routinely used in immune-based assays. Thus, the DBA/2 mouse is well employed in the field of immunology and their cells (like these DBA/2 Mouse PBMCs) may offer experimental advantages depending on the parameters of your experiments.
Application Summary for DBA/2 Mouse PBMCs
DBA/2 Mouse PBMCs can be used for a wide variety of immunology-based applications. In-vitro immunoassays, such as ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to measure ADCC or CDC, and plaque assays are routinely performed using these cells. In addition, PBMCs are also used in ex-vivo applications, such as cell population characterization and generation of genetically modified cells through retroviral infection.
Isolation of PBMCs
Peripheral blood is obtained from responsible facilities that have many years of experience collecting samples. Since the facilities are local, the blood is quickly transported to iQ Bioscience’s laboratory for processing.
Once at our lab, PBMCs are isolated by performing density centrifugation with Ficoll, a high molecular weight sucrose solution, to remove red blood cells under sterile conditions. Depending on the species (human or non-human) of blood, either 1.077 or 1.084 g/ml Ficoll is used, resulting in a perfect layer of mononuclear cells that are separated from plasma, platelets, granulocytes, and erythrocytes. Additional quality control steps are taken to isolate and prepare PBMCs that ensure the highest viability for cryopreservation and downstream experimental applications.
Cryopreservation and Storage
Our mouse PBMCs are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 80%) after thawing.
Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).