Name: Wistar Rat Peripheral Blood Mononuclear Cells (PBMCs)
SKU: IQB-RPB2xx
Availability: InStock

Wistar Rat Peripheral Blood Mononuclear Cells (PBMCs)

$240.00 – $400.00

High-quality PBMCs from the Wistar rat, a widely-used outbred rat strain
High viability (> 80%) after thawing
Used for a wide variety of immunology-based applications, including in-vitro immunoassays and ex-vivo applications
All orders come with an iQ Certificate of Analysis
Normally ships out same business day

SKU: IQB-RPB2xx Categories: PBMC, Rat, Wistar Tags: pbmc, Rat, wistar

Description

About the Wistar Rat

The Wistar rat is one of the two most routinely-used outbred albino rat strains in biomedical research. They are commonly used in many fields of research, including oncology, aging, pharmacology, toxicology, and metabolism/nutrition. They are also used in immunology as models for infectious diseases and autoimmune responses. Depending on the parameters of your experiments, cells from Wistar rats (like Wistar rat PBMCs) may offer experimental advantages.

Application Summary for Wistar Rat PBMCs

Rat PBMCs can be used for a wide variety of immunology-based applications. In-vitro immunoassays, such as ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to measure ADCC or CDC, and plaque assays are routinely performed using these cells. In addition, rat PBMCs are also used in ex-vivo applications, such as cell population characterization and generation of genetically modified cells through retroviral infection.

Isolation of PBMCs

Peripheral blood is obtained from responsible facilities that have many years of experience collecting samples. Since the facilities are local, the blood is quickly transported to iQ Bioscience’s laboratory for processing.

Once at our lab, PBMCs are isolated by performing density centrifugation with Ficoll, a high molecular weight sucrose solution, to remove red blood cells under sterile conditions. Depending on the species (human or non-human) of blood, either 1.077 or 1.084 g/ml Ficoll is used, resulting in a perfect layer of mononuclear cells that are separated from plasma, platelets, granulocytes, and erythrocytes. Additional quality control steps are taken to isolate and prepare PBMCs that ensure the highest viability for cryopreservation and downstream experimental applications.

Cryopreservation and Storage

Our rat PBMCs are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 80%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).

Additional information

Available Size(s)	10 million cells / vial, 5 million cells / vial
Cell Type	Mononuclear
Format	Frozen
Species	Rat
Tissue Type	Normal Peripheral Blood
Viability	> 80%

For US customers, we ship via FedEx Overnight Shipping. Shipping charges will vary per shipping address (based on ZIP code) and are estimated to be $110.

For international (non-US) customers, we work closely with you and our couriers to ensure all necessary documentation is in place for international shipments to significantly reduce the chance of delays at Customs. For the export of non-human primate samples, this includes preparing CITES permits, as well as any other documentation as required by country. Please submit an inquiry to orders@iqbiosciences.com for your estimated time of delivery and shipping charges.

Austria

Hölzel Diagnostika Handels GmbH
Tel: +49 221 126 02 66
Email: info@hoelzel.de
Web: https://www.hoelzel-biotech.com/

Canada

Cedarlane
Tel: +1 (289) 288-0001
Toll Free (North America): +1 (800) 268-5058
Fax: +1 (289) 288-0020
Email: sales@cedarlanelabs.com
Web: https://www.cedarlanelabs.com

China

BIOHUB INTERNATIONAL TRADE CO., LTD.
上海起发实验试剂有限公司
Address: Chuansha Rd #6619, Pudong, Shanghai, Zipcode: 201200 P.R.China
Tel: 0086-021-50724187
Phone: +86-15921799099
Fax: 0086-021-50724961
Email: sale3@78bio.com
Web: www.qfbio.com

European Union