Description

About the Rhesus Monkey (or Rhesus Macaque)

Rhesus macaques are one of the most commonly utilized non-human primates in biomedical research. They are employed in numerous research areas, such as immunology, neuroscience, oncology, infectious disease, and toxicology due to their physiology.

Application Summary for Rhesus Macaque Splenocytes

Rhesus macaque splenocytes can be used for a wide variety of safety assessment and functional studies. They are commonly employed in pre-clinical settings to ensure biologics are not eliciting unwanted functions, such as cytokine release and toxicity. They are also used in many immunology-based applications, such as ELISPOT, cytokine production assays, and cytotoxicity assays to measure ADCC or CDC in response to biologics. Rhesus macaque splenocytes can also be used in ex-vivo applications for cell population characterization. They can also be used to test the toxicity of compounds.

Isolation of Rhesus Macaque Splenocytes

Spleens from rhesus macaques are collected responsibly at third party facilities. These facilities are vetted to ensure they operate according to local regulations and laws. Since the facility is local, the tissue is quickly transported to iQ’s facility for processing.

At our facility, the splenic tissue is carefully dissociated through a gentle mechanical process to generate a single cell suspension under sterile conditions. The suspension is then carefully passed through a filter to remove any fat and/or connective tissue and subsequently depleted of red blood cells in a manner that is not damaging to the other cells. The purified splenocytes are then frozen based on iQ Biosciences’ quality-controlled cryopreservation protocol to promote recoverability and viability once in the end user’s hands.

Cryopreservation and Storage

The rhesus macaque splenocytes are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (typically > 70%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).