- High-quality PBMCs from the Sprague Dawley rat, a commonly-used outbred rat strain
- High viability (> 80%) after thawing
- Used for a wide variety of immunology-based applications, including in-vitro immunoassays and ex-vivo applications
- All orders come with an iQ Certificate of Analysis
- Normally ships out same business day
SD Rat Peripheral Blood Mononuclear Cells (PBMCs)
$225.00 – $375.00
About the Sprague Dawley Rat
The Sprague Dawley rat is one of the two most widely-used outbred rat strains in biomedical research, including oncology, neurobiology, toxicology, pharmacology, aging, metabolism/nutrition, and neurobiology. Derived from the Wistar rat strain, the Sprague Dawley rat is also routinely employed in the field of immunology research as a model for autoimmune diseases and immunogenic challenges against pathogens. Thus, cells from Sprague Dawley rats (like SD rat PBMCs) may offer experimental advantages depending on the parameters of your experiments.
Application Summary for SD Rat PBMCs
Rat PBMCs can be used for a wide variety of immunology-based applications. In-vitro immunoassays, such as ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to measure ADCC or CDC, and plaque assays are routinely performed using these cells. In addition, rat PBMCs are also used in ex-vivo applications, such as cell population characterization and generation of genetically modified cells through retroviral infection.
Isolation of PBMCs
Peripheral blood is obtained from responsible facilities that have many years of experience collecting samples. Since the facilities are local, the blood is quickly transported to iQ Bioscience’s laboratory for processing.
Once at our lab, PBMCs are isolated by performing density centrifugation with Ficoll, a high molecular weight sucrose solution, to remove red blood cells under sterile conditions. Depending on the species (human or non-human) of blood, either 1.077 or 1.084 g/ml Ficoll is used, resulting in a perfect layer of mononuclear cells that are separated from plasma, platelets, granulocytes, and erythrocytes. Additional quality control steps are taken to isolate and prepare PBMCs that ensure the highest viability for cryopreservation and downstream experimental applications.
Cryopreservation and Storage
Our rat PBMCs are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (> 80%) after thawing.
Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).
For US customers, we ship via FedEx Overnight Shipping. Shipping charges will vary per shipping address (based on ZIP code) and are estimated to be $110.
For international (non-US) customers, we work closely with you and our couriers to ensure all necessary documentation is in place for international shipments to significantly reduce the chance of delays at Customs. For the export of non-human primate samples, this includes preparing CITES permits, as well as any other documentation as required by country. Please submit an inquiry to email@example.com for your estimated time of delivery and shipping charges.