- High-quality splenocytes from the DBA/2 mouse, a widely-used inbred mouse strain
- High viability (typically > 70%) after thawing
- Used for a wide variety of immunology-based applications, including in-vitro immunoassays and ex-vivo applications
- All orders come with an iQ Certificate of Analysis
- Normally ships out same business day
DBA/2 Mouse Splenocytes
$600.00 – $900.00
About the DBA/2 Mouse
The DBA/2 mouse is a widely-used inbred mouse strain to study cardiovascular biology, neurobiology, and immunology. Often contrasted to the C57BL/6 mouse, the DBA/2 mouse carries the H-2d haplotype and their immune cells are routinely used in immune-based assays. Thus, the DBA/2 mouse is well employed in the field of immunology and their cells (DBA/2 mouse splenocytes) may offer experimental advantages depending on the parameters of your experiments.
Application Summary for DBA/2 Mouse Splenocytes
DBA/2 mouse splenocytes can be used for a wide variety of immunology-based applications. In-vitro immunoassays, such as ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to measure ADCC or CDC, and plaque assays are routinely performed using these cells. In addition, mouse splenocytes are also used in ex-vivo applications, such as cell population characterization and generation of genetically modified cells through retroviral infection.
Isolation of Splenocytes
Spleen Collection and Splenocyte Isolation
Spleens are isolated responsibly from DBA/2 mice at third party facilities. These facilities are vetted to ensure they operate according to local regulations and laws. Since the facility is local, the tissue is quickly transported to iQ’s facility for processing.
Once at our facility, the splenic tissue is carefully dissociated through a gentle mechanical process to generate a single cell suspension under sterile conditions. The suspension is then carefully passed through a filter to remove any fat and/or connective tissue and subsequently depleted of red blood cells in a manner that is not damaging to the other cells. The purified splenocytes are then frozen based on iQ Biosciences’ quality-controlled cryopreservation protocol to promote recoverability and viability once in the end user’s hands.
Cryopreservation and Storage
Our DBA/2 mouse splenocytes are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (typically > 70%) after thawing.
Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).
Typically > 70%
For US customers, we ship via FedEx Overnight Shipping. Shipping charges will vary per shipping address (based on ZIP code) and are estimated to be $110.
For international (non-US) customers, we work closely with you and our couriers to ensure all necessary documentation is in place for international shipments to significantly reduce the chance of delays at Customs. For the export of non-human primate samples, this includes preparing CITES permits, as well as any other documentation as required by country. Please submit an inquiry to email@example.com for your estimated time of delivery and shipping charges.
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