DBA/2 Mouse Splenocytes

$600.00$900.00

Features

  • High-quality splenocytes from the DBA/2 mouse, a widely-used inbred mouse strain
  • High viability (typically > 70%) after thawing
  • Used for a wide variety of immunology-based applications, including in-vitro immunoassays and ex-vivo applications
  • All orders come with an iQ Certificate of Analysis
  • Normally ships out same business day

Contact us for info on bulk discounts

Description

About the DBA/2 Mouse

The DBA/2 mouse is a widely-used inbred mouse strain to study cardiovascular biology, neurobiology, and immunology. Often contrasted to the C57BL/6 mouse, the DBA/2 mouse carries the H-2d haplotype and their immune cells are routinely used in immune-based assays. Thus, the DBA/2 mouse is well employed in the field of immunology and their cells (DBA/2 mouse splenocytes) may offer experimental advantages depending on the parameters of your experiments.

Application Summary for DBA/2 Mouse Splenocytes

DBA/2 mouse splenocytes can be used for a wide variety of immunology-based applications. In-vitro immunoassays, such as ELISPOT, mixed lymphocyte reactions, cytotoxicity assays to measure ADCC or CDC, and plaque assays are routinely performed using these cells. In addition, mouse splenocytes are also used in ex-vivo applications, such as cell population characterization and generation of genetically modified cells through retroviral infection.

Isolation of Splenocytes

Spleen Collection and Splenocyte Isolation

Spleens are isolated responsibly from DBA/2 mice at third party facilities. These facilities are vetted to ensure they operate according to local regulations and laws. Since the facility is local, the tissue is quickly transported to iQ’s facility for processing.

Once at our facility, the splenic tissue is carefully dissociated through a gentle mechanical process to generate a single cell suspension under sterile conditions. The suspension is then carefully passed through a filter to remove any fat and/or connective tissue and subsequently depleted of red blood cells in a manner that is not damaging to the other cells. The purified splenocytes are then frozen based on iQ Biosciences’ quality-controlled cryopreservation protocol to promote recoverability and viability once in the end user’s hands.

Cryopreservation and Storage

Our DBA/2 mouse splenocytes are cryopreserved carefully using iQ Biosciences’ cryopreservation protocol that ensures high viability (typically > 70%) after thawing.

Cells should be stored at < -120°C once they are received, such as within a liquid nitrogen tank (vapor phase).

Additional information

Available Size(s)

Cell Type

Format

Species

Tissue Type

Viability

Typically > 70%

Shipping Information

For US customers, we ship via FedEx Overnight Shipping. Shipping charges will vary per shipping address (based on ZIP code) and are estimated to be $140.

For international (non-US) customers, we work closely with you and our couriers to ensure all necessary documentation is in place for international shipments to significantly reduce the chance of delays at Customs. For the export of non-human primate samples, this includes preparing CITES permits, as well as any other documentation as required by country. Please submit an inquiry to orders@iqbiosciences.com for your estimated time of delivery and shipping charges.

Distributors

Austria Austria FlagHölzel Diagnostika Handels GmbH

+49 221 126
Canada Canada flagCedarlane

+1 (289) 288-0001
+1 (800) 268-5058
+1 (289) 288-0020
China China FlagBIOHUB INTERNATIONAL TRADE CO., LTD. 上海起发实验试剂有限公司
Address: Chuansha Rd #6619, Pudong, Shanghai, Zipcode: 201200 P.R.China

0086-021-50724187
+86-15921799099
0086-021-50724961
GermanyGermany FlagHölzel Diagnostika Handels GmbH

+49 221 126 02 66

Zageno

Ireland Ireland Flag 2B Scientific Ltd

+44(0) 1869 238033
+44(0) 1869 238034
India India FlagCell & Gene BioSolutions Pvt. Ltd. #478 C, SLV Complex, Raghavendra Swamy Mutt Road Opp. Turahalli Water
Tank, Turahalli, Subramanyapura Post Uttarahalli Hobli, Bengaluru-560061, Karnataka, India

+91 97317 14670
+91 98809 25033
JapanJapan FlagContacts:

+81 (03) 5632 9610
+81 (03) 5632 9619
Qatar Singapore FlagSedeer Medical Services and Trading LLC

+974 4434 9191
Singapore Singapore FlagOmnicell Pte Ltd

+65 6747 0201
South Korea South Korea FlagBioClone

+82-2-2690-0058
Switzerland Austria Flag Hölzel Diagnostika Handels GmbH

+49 221 126 02 66
United States United States Flag Fisher Scientific

1-800-766-7000

Quartzy

VWR International

1-800-932-5000

Zageno

United Kingdom United Kingdom Flag 2B Scientific Ltd

+44(0) 1869 38033
+44(0) 1869 238034

 https://www.2bscientific.com
Caltag Medsystems Ltd.

+44 (0)1280 827460
+44 (0)1280 827466

Tebubio

+44 (0)1733 421880
+44 (0)1733 421882

 https://www.tebubio.com
Zageno

Taiwan Taiwan Flag Hycell International Co. Ltd.

Ordering Process

Step 1

Frame 1000003682
Search for products online

Access our easy-to-navigate catalog and find the cell types from the species you’re looking for. Add 
your desired quantities to the cart.

Step 2

checkout
Checkout

Once you’re done shopping, complete the easy checkout process. We accept credit card payments for immediate billing, as well as purchase orders via file upload.

Step 3

receive
Receive your product – FAST!

For your convenience, most of our products ship the same day with overnight delivery, as long as your order is submitted by 2PM Pacific Time Monday – Thursday. Be prepared to receive your cryopreserved product.

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